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pYPQ141D2.0

Plasmid

#99906

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Rfx4

Plasmid

#61544

Purpose

Expresses Rfx4 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Rfx4 (Rfx4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pCS2-Opto-Alk3

Plasmid

#207614

Purpose

Zebrafish Alk3 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Vaucheria frigida, Zebrafish)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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10xUAS-GFP-DD

Plasmid

#104989

Purpose

Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GFP (GFP-DD)

Tags

DD

Expression

Insect

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdTomato-WPRE

Plasmid

#104105

Purpose

Expresses tdTomato under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tdTomato

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJC25NM

Plasmid

#1126

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

NM stop (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pAAV.hSynap.(cyto).iGlucoSnFR2

Plasmid

#244091

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H2B-mAvicFP1

Plasmid

#129511

Purpose

Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

H2B-mAvicFP1

Expression

Mammalian

Mutation

mAvicFP1 fused to the C terminus of human histone…

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_002

Plasmid

#123960

Purpose

Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - 2xHS4 - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_043

Plasmid

#123943

Purpose

Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - A1 Insulator - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ74-NLS

Plasmid

#231892

Purpose

Forms nuclear HTT partial exon 1 Q74-NLS aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74-NLS (HTT Human)

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-Nir1-LNS2

Plasmid

#220084

Purpose

high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM3 (PITPNM3 Human)

Tags

mCherry-GGSGGM

Expression

Mammalian

Mutation

amino acids 613-897

Promoter

CMV

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLK

Plasmid

#201584

Purpose

Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP9A-HA

Plasmid

#209213

Purpose

Mammalian expression of ATP9A

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP9A (ATP9A Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPBT-PE2-PGK-Blast

Plasmid

#173219

Purpose

piggyBac transposon containing PE2 and Blasticidin expression cassettes

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

PE2
Blast

Use

Piggybac

Expression

Mammalian

Promoter

CMV and hPGK

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

Plasmid

#179585

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NLS-NG-T2A

Promoter

CAGp

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCK01_PalkS_alkS

Plasmid

#166502

Purpose

Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

alkS

Use

Synthetic Biology

Expression

Bacterial

Mutation

L401G

Promoter

PalkS

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAB_PalkB_sfgfp_KanR

Plasmid

#166503

Purpose

Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PalkB

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Plasmid EUPp_09

Plasmid

#157745

Purpose

PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

Depositor

Kang Zhou

Article

Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

Inserts

acetaldehyde dehydrogenase
alcohol dehydrogenase (ADH2 Budding Yeast)

Expression

Bacterial

Promoter

PgyrA

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9037

Plasmid

#153225

Purpose

Template for PCR amplification of the constant part of guide RNAs

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

guide RNA constant part, carotenoid marker

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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