We narrowed to 11,646 results for: emb
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Plasmid#173853PurposeProtein expression plasmid for recombinant His-Myc tag mouse PLD3DepositorInsertPLD3 (Pld3 Mouse)
Tags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI
Plasmid#140202PurposeCRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.DepositorInsertmCherry
UseCRISPR and Synthetic Biology; Proof-of-concept te…MutationG174DPromoterPelcAAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1mut
Plasmid#86690PurposeFor transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)
TagsHAMutationmutated region NHDC (asparagine 273 to cysteine 2…PromoterT3Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
LRRC8b-His-bio
Plasmid#53362PurposeExpresses full-length Leucine rich repeat containing 8 family, member B ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertLRRC8b (LRRC8B Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CLEC1B-His-bio
Plasmid#52329PurposeExpresses full-length C-type lectin domain family 1 member B ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertCLEC1B (CLEC1B Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-mCherry
Plasmid#50480Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsmCherry fusion in M3-M4 loop (after residue A405)ExpressionMammalianPromoterCMVAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5/FRT/TO DNAJB2b
Plasmid#19470DepositorInsertDNAJB2b (DNAJB2 Human)
ExpressionMammalianAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJA4
Plasmid#19548DepositorAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
InterTag-BFP
Plasmid#186901PurposeCell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTagDepositorInsertSpyTag003-sfCherry11-tagBFP-PDGFR
UseSynthetic BiologyAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-GFP
Plasmid#186900PurposeCell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10DepositorInsertTfR-sfGFP-SpyCatcher003-sfCherry1-10
UseSynthetic BiologyAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
BASIC_5_Amp
Plasmid#68139Purposestorage vector for BASIC part: AmpDepositorPublicationInsertAmpicillin
ExpressionBacterialAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
BASIC_1_MB1
Plasmid#68135Purposestorage vector for BASIC part: MB1DepositorPublicationInsertMB1 origin
ExpressionBacterialAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax
Plasmid#190153PurposeExpresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp2 (Vamp2 Mouse)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only AA #1-94PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD40-bio-His
Plasmid#51648PurposeExpresses full-length Tumor necrosis factor receptor superfamily member CD40 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD40 molecule (CD40 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
nes374tk/lacZ
Plasmid#47615Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron fragment (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZMutation374 bp from 2nd intron (comprising bp 1153 to 152…PromoterHSV tk promoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRDA_831
Plasmid#216100PurposeCRISPRa, EF1a-driven dCas9-VP64 (Cas only)DepositorInsertCas9 [Sp]
UseCRISPR and Lentiviral; Assembled vectorAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAraTM-2BTMCYL980A
Plasmid#39550DepositorPublicationInsertIntegrin alpha2B L980A TM-CYTO
ExpressionBacterialPromoterTacAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only