We narrowed to 9,400 results for: Coli

1. pAW-SpyCas2

   Plasmid

   #113249

   Purpose

   E. coli expression vector for His-MBP-S. pyogenes Cas2

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   S. pyogenes Cas2
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRD324

   Plasmid

   #198045

   Purpose

   Expression of HMfB D14A K30A E34A in E. coli

   Depositor

   Remus Dame

   Article

   Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

   Insert

   HMfB D14A K30A E34A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet11a-ABLE

   Plasmid

   #158627

   Purpose

   Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

   Depositor

   William DeGrado

   Article

   Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

   Insert

   Apixaban-Binding Helical Bundle
   

   Use

   Tags

   6x His-tag and TEV cleavage tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcrRNA.ind

   Plasmid

   #61284

   Purpose

   Arabinose-inducible repeat-only E. coli CRISPR array

   Depositor

   Chase Beisel

   Article

   Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT105

   Plasmid

   #25089

   Depositor

   Christopher Voigt

   Article

   Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

   Insert

   Edge detector delta cI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-c5X-ELP[V5A2G3-60]

   Plasmid

   #67009

   Purpose

   Expresses malE-ELP[V5A2G3-60] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-ELP[V5A2G3-60]
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDW114

   Plasmid

   #70061

   Purpose

   artificial transcription template "wild-type"

   Depositor

   Jeff Gelles

   Article

   Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

   Insert

   lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGEX-6P-1-GST-CD-ATL (Cbx7)

   Plasmid

   #82527

   Purpose

   Expresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 CD-ATL region, amino acids 1–84.

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 7 (CBX7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1–84

   Promoter

   Available Since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEXndoE

   Plasmid

   #47714

   Purpose

   Expresses GST-EndoE from E. faecalis in E. coli

   Depositor

   Mattias Collin, Vincent Fischetti

   Article

   Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

   Insert

   ndoE
   

   Use

   Tags

   Glutathione S-transferase

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHL 2057

    Plasmid

    #64909

    Purpose

    AmpR + ColE1 + PLtetO-1::RBS(st7):cro::cII (31 basepairs)::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator

    Depositor

    Han Lim

    Article

    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pUC57-FASTKD4

    Plasmid

    #73620

    Purpose

    Cloning vector containing FASTKD4

    Depositor

    Vamsi Mootha

    Article

    Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

    Insert

    FASTKD4 (TBRG4 Human)
    

    Use

    Tags

    Expression

    Mutation

    FASTKD4 has been codon optimized for expression i…

    Promoter

    lac

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pHL 2067

    Plasmid

    #64910

    Purpose

    AmpR + ColE1 + PLtetO-1:: extra 5’UTR::RBS(st7):cfp::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator

    Depositor

    Han Lim

    Article

    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pPLP1.3

    Plasmid

    #22615

    Depositor

    Lynn Hudson

    Article

    Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

    Insert

    Proteolipid Protein
    

    Use

    E. coli phagemid vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

14. I766T USP7 UBL-12

    Plasmid

    #227232

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    I766T; aa 535-775

    Promoter

    T7-promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. L757P USP7 UBL-12

    Plasmid

    #227231

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L757P; aa 535-775

    Promoter

    T7-promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28_Strep-Psc-pScaC

    Plasmid

    #228834

    Purpose

    E. coli expression of the ScaC passenger domain

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 33-223

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_pScaC(50-159)

    Plasmid

    #228836

    Purpose

    E. coli expression of a truncated ScaC passenger domain (aa 50-159)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 50-159

    Promoter

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R152C USP7 TRAF

    Plasmid

    #227229

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    R152C; aa 62-205

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. G392A FL USP7

    Plasmid

    #227217

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392A

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L469R USP7 catalytic

    Plasmid

    #227212

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L469R; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET27b+R

    Plasmid

    #188245

    Purpose

    Empty backbone vector for protein expression in Escherichia coli and his-tag purification

    Depositor

    Yiping Qi

    Article

    Fang et al Methods Mol Biol. 2023;2653:153-171. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJExpress401-T4 Lysozyme C54T C97A (WT*)

    Plasmid

    #224353

    Purpose

    Expresses T4 lysozyme in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al MethodsX. 2015 May 18;2:256-62. doi: 10.

    Insert

    T4 Lysozyme
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    C45T, C94A

    Promoter

    T5

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pBz84

    Plasmid

    #226318

    Purpose

    E. coli protein expression for protein purification

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    6xHis- (wtMR1-6)- strep
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    N-terminal domain deleted, C-terminal domain dele…

    Promoter

    T7

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. N418S FL USP7

    Plasmid

    #227215

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    N418S

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. H111Q USP7 TRAF

    Plasmid

    #227227

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    H111Q; aa 62-205

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. G392D FL USP7

    Plasmid

    #227216

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392D

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. K420E FL USP7

    Plasmid

    #227223

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    K420E

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. L299A USP7 catalytic

    Plasmid

    #227213

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L299A; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. V485G USP7 catalytic

    Plasmid

    #227209

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    V485G; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. P273H USP7 catalytic

    Plasmid

    #227208

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    P273H; aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge