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Addgene

We narrowed to 13,015 results for: cdna

Showing: 7601 - 7640 of 13015 results
  1. RDX-mCherry

    Plasmid
    #214476
    Purpose
    Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.
    Depositor
    Insert
    RDX (RDX Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Sequence matches cDNA clone MGC:48283 IMAGE:52844…
    Promoter
    CMV
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid
    #224898
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
    Depositor
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pMTMVi-M

    Plasmid
    #118757
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.
    Depositor
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pAC-BETA-At

    Plasmid
    #53288
    Purpose
    Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and an lcyB cDNA of Arabidopsis thaliana. Use with pY2F to produce alpha-carotene in E. coli.
    Insert
    lcyB (LYC Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pAC-aZEA

    Plasmid
    #53286
    Purpose
    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli
    Insert
    lcyE (LUT2 Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lac
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pLEG(R1-R3) #BGV149

    Plasmid
    #48956
    Purpose
    Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. G protein alpha-s-HaloTag

    Plasmid
    #66993
    Purpose
    G protein alpha-s internally tagged with HaloTag and EE epitope
    Depositor
    Insert
    G-alpha-s-EE-HaloTag (Gnas Rat)
    Use
    Tags
    HaloTag flanked by SGGGGSGGGGS on each side was i…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pMTMVi-N

    Plasmid
    #118756
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.
    Depositor
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pBSFI-myr-akt-S473A

    Plasmid
    #49190
    Purpose
    cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector
    Depositor
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Adaptor plasmid
    Tags
    HA tag and myristolation signal
    Expression
    Mutation
    S473A
    Promoter
    T7
    Available since
    Dec. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
  10. pBSFI-myr-akt-T308A

    Plasmid
    #49189
    Purpose
    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector
    Depositor
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Adaptor plasmid
    Tags
    HA tag and myristolation signal
    Expression
    Mutation
    T308A
    Promoter
    T7
    Available since
    Dec. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
  11. pREG(R1-R3) #BGV141

    Plasmid
    #48958
    Purpose
    Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Depositor
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

    Plasmid
    #124299
    Purpose
    Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Insert
    UBE2G1 (UBE2G1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pJL3

    Plasmid
    #184131
    Purpose
    For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.
    Depositor
    Insert
    MRP-1 (mrp-1 Nematode)
    Use
    Tags
    GFP
    Expression
    Worm
    Mutation
    Promoter
    Pges-1 (3.3kb)
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pLVX-IRES-tdTomato-FlagAkt2W80A

    Plasmid
    #64834
    Purpose
    cDNA of Akt2 with point mutation from Trp to Ala at position 80. This mutation confers resistance to MK-2206 drug.
    Depositor
    Insert
    Akt2 (Akt2 Mouse)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Point mutation Trp 80 to Ala
    Promoter
    CMV
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 7601 - 7640 of 13015 results