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We narrowed to 12,112 results for: LEA

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Showing: 3801 - 3820 of 12112 results

  1. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

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  3. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid
    #193253
    Purpose
    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Depositor
    Mark Sellmyer
    Publication
    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1
    Insert
    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Use
    Lentiviral
    Tags
    Renilla luciferase (rLuc) and yellow fluorescent …
    Mutation
    None
    Promoter
    EF1a
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    193253-plasmid-map-sequence-id-383064

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  4. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184248-plasmid-map-sequence-id-370322

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  6. pcDNA3-RsSHC014-RBD-8h

    Plasmid
    #161826
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161826-plasmid-map-sequence-id-316777

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  7. pcDNA3-Rs7327-RBD-8h

    Plasmid
    #161823
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161823-plasmid-map-sequence-id-316772

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  8. pCEP4-HA-Flag-natT1R2 FS1

    Plasmid
    #113955
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, one base pair dele…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113955-plasmid-map-sequence-id-215169

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  10. mCherry-CRY2-P14P5K

    Plasmid
    #79570
    Purpose
    expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c
    Depositor
    Olof Idevall-Hagren
    Publication
    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26
    Insert
    Pip5k1c (Pip5k1c Mouse)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    R445E and R446E; truncation of amino acids 636-66…
    Promoter
    CMV
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pCMV-MMLV-gag-hMLH1dn

    Plasmid
    #195513
    Purpose
    MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.
    Depositor
    Hyongbum Kim
    Publication
    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.
    Insert
    hMLH1dn (MLH1 Human)
    Use
    Virus like particle
    Tags
    FLAG, NES, and Protease cleavate site
    Expression
    Mammalian
    Mutation
    Human MLH1 del754-756
    Promoter
    CMV
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195513-plasmid-map-sequence-id-384690

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  18. ATX3-77Q

    Plasmid
    #184249
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Codon optimization for protein expression in BL2…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184249-plasmid-map-sequence-id-370367

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  19. pGBW-m4046942

    Plasmid
    #145757
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145757-plasmid-map-sequence-id-292503

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  20. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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Showing: 3801 - 3820 of 12112 results