We narrowed to 9,779 results for: virus
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Plasmid#196582PurposeMammalian expression of SpCas9 prime editor 2 with P2A-p53DDDepositorInsertPE2-P2A-mp53DD
ExpressionMammalianMutationΔ40-903PromoterCMVAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Nuclease-GFP
Plasmid#171994PurposeDelivers all prime editing nuclease components in a single, GFP selectable plasmidDepositorInsertCbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
ExpressionMammalianMutationGC to CA point mutation in SpCas9 to restore nucl…PromoterCMV for Cas9, U6 for gRNAsAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcsII-pb4-flag
Plasmid#86768PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertproteasome beta subunit 4 (PSMB4 Human)
UseLentiviralTagsflagExpressionMammalianPromoterEF-1aAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTNG4kwh
Plasmid#44722DepositorInsertspCMV-D2i promoter
htetR::NLS::EGFP
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Twin-Strep-TEV-DGKζ
Plasmid#202531PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cellDepositorInsertDGKZ (DGKZ Human)
TagsTEV cleavable site and Twin-Strep-tagExpressionMammalianPromoterCAG and chicken β-actin promoterAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD
Plasmid#214084PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-hP53DDDepositorInsertPEmax-P2A-hP53DD (TP53 Synthetic, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Human)
ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro
Plasmid#120560PurposeExpresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus productionDepositorInsertHRAS (HRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationG12VPromoterCMVAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA
Plasmid#113692PurposeU6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsNLSPromoterCytomegalo Virus(CMV)Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-Kras-Blasticidin
Plasmid#120714PurposeExpresses membrane-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertKRAS4B (KRAS Human)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationamino acids 158-188PromoterCMVAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgAAVS1(A)
Plasmid#85570PurposeExpresses sgRNA targeting human AAVS1 locusDepositorInsertadeno-associated virus integration site 1 (AAVS1 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgAAVS1(B)
Plasmid#85571PurposeExpresses sgRNA targeting human AAVS1 locusDepositorInsertadeno-associated virus integration site 1 (AAVS1 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113687PurposeSaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS
Plasmid#85734PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272DepositorInsertETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)
Tags6xHis following thrombin cleavage site LVPR|GExpressionBacterialAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113688PurposeSaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-CXCR4-VN
Plasmid#98964PurposeFLAG-tagged human CXCR4 fused to N-terminus of split VenusDepositorInsertCXCR4 (CXCR4 Human)
TagsFLAG (dykdddd) epitope tag, Signal/leader sequenc…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-CXCR4-VC
Plasmid#98967Purposemyc-tagged human CXCR4 fused to C-terminus of split VenusDepositorInsertCXCR4 (CXCR4 Human)
TagsSignal/leader sequence from Influenza A virus HA …ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (del.Cyto)
Plasmid#49209Purposeexpresses Flag tagged mouse Tim3 fragment (no cytosolic domain)DepositorInsertTim3 (del.Cyto) (Havcr2 Mouse)
TagsCD8 signal sequence and FLAGExpressionMammalianMutationcytosolic domain deleted, no Tim3 signal sequence…PromoterEf1aAvailable SinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (T2 truncation)
Plasmid#49211Purposeexpresses Flag tagged mouse Tim3 (T2 truncation)DepositorInsertTim3 T2 truncation (Havcr2 Mouse)
TagsFLAG and signal sequenceExpressionMammalianMutationT2 truncation ( lacks all but one tyrosine, which…PromoterEF1aAvailable SinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (T1 truncation)
Plasmid#49210Purposeexpresses Flag tagged mouse Tim3 (T1 truncation)DepositorInsertTim3 T1 truncation (Havcr2 Mouse)
TagsFLAG and signal sequenceExpressionMammalianMutationT1 truncation ( contains all but the three most C…PromoterEF1aAvailable SinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLK06Hpp-Fab CR3009
Plasmid#239861PurposePlasmid for simultaneous Fab and GFP expression from separate Lac promoters for online monitoring of induction with a GFP sensor station. Fab is expressed as a fusion with alkaline phosphatase.DepositorInsertsTagsHexahistidine tag and bacterial alkaline phosphat…ExpressionBacterialMutationF64L, S65T, Q80R, M153T and V163APromoterLac promoterAvailable SinceSept. 5, 2025AvailabilityAcademic Institutions and Nonprofits only