We narrowed to 29,518 results for: REP

1. pAAV CaMKIIa iChloC 2A tDimer

   Plasmid

   #66709

   Purpose

   Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

   Depositor

   Thomas Oertner

   Article

   Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E83Q,E90R,E101S,D156N,T159C

   Promoter

   CaMKIIa

   Available since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRH051

   Plasmid

   #171625

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BsmBI-Destination
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDN34-BiNLS-hYAP1-1delta

   Plasmid

   #169471

   Purpose

   expresses optoYAP in mammalian cells

   Depositor

   Timothy Saunders

   Article

   Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

   Insert

   YAP1-1delta (YAP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-CUL4A-pCMV7.1

   Plasmid

   #155022

   Purpose

   N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 4A (CUL4A Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAD-KPS12-Pman

   Plasmid

   #133832

   Purpose

   Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   mKate(KPS12)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pman

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-UniSAM

   Plasmid

   #99866

   Purpose

   Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

   Depositor

   Lesley Forrester

   Article

   Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

   Insert

   UniSAM-mCherry + U6-gRNA2.0
   

   Use

   CRISPR and Synthetic Biology; Dcas9-sam activation

   Tags

   Expression

   Mammalian

   Mutation

   BbsI sites in CDS were ablated by consensus mutat…

   Promoter

   EF1a

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CLYBL-TO-PAX7

   Plasmid

   #204346

   Purpose

   Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   human PAX7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-NLS-CBRed-d2-hygro

   Plasmid

   #139196

   Purpose

   non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBRed
   

   Use

   Luciferase and Retroviral

   Tags

   d2 PEST domain and nuclear localization signal (N…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.10-VEGFprom(-1000 to -1)

   Plasmid

   #66128

   Purpose

   Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

   Depositor

   David Mu

   Article

   Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

   Insert

   Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   VEGF -1000 to -1

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. -5.5kb-UCP1-GFP

    Plasmid

    #104585

    Purpose

    Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

    Depositor

    Irfan Lodhi

    Article

    Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

    Insert

    UCP1 promoter (Ucp1 Mouse)
    

    Use

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UCP1

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-EGFP-WPRE

    Plasmid

    #187103

    Purpose

    Double floxed EGFP under the control of EF1a promoter

    Depositor

    Yongling Zhu

    Article

    Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-TORCAR(T/A)

    Plasmid

    #64928

    Purpose

    Negative-control mutant for TORCAR biosensor.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR(T/A) (EIF4EBP1 Human, Synthetic)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    4EBP1 domain contains T37A and T46A mutations.

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. eGFP-KIF5B-Rigor

    Plasmid

    #172204

    Purpose

    Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-IRES-Hygro_RGECO1.2

    Plasmid

    #164592

    Purpose

    Stable integration and expression of RGECO1.2 in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    R-GECO1.2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SuperNova/pRSETB

    Plasmid

    #53234

    Purpose

    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

    Depositor

    Takeharu Nagai

    Article

    Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

    Insert

    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
    

    Use

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-mTagBFP2-P2A-EGFP

    Plasmid

    #184943

    Purpose

    Expresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    mTagBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

    Plasmid

    #158967

    Purpose

    Ubiquitous expression of mtON in C. elegans

    Depositor

    Andrew Wojtovich

    Article

    Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

    Insert

    eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    eft-3

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Green Pegassos

    Plasmid

    #163114

    Purpose

    Expresses biosensors for pyruvate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Pegassos
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP_WT-talin1 (1-2541)

    Plasmid

    #166112

    Purpose

    For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    WT-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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