We narrowed to 16,979 results for: She

1. pLD-puro-Cc-IFIT5_K160R-VA

   Plasmid

   #191222

   Purpose

   Lentiviral expression of human IFIT5_K160R in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   changed Lysine 160 to Arginine (K160R)

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXs-hNeuroD1

   Plasmid

   #233184

   Purpose

   Retroviral expression of NeuroD1

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

   Insert

   NeuroD1 (NEUROD1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXs-mBrn2

   Plasmid

   #233151

   Purpose

   Retroviral expression of Brn2

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMXs-hMyt1l

   Plasmid

   #233153

   Purpose

   Retroviral expression of Myt1l

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

   Insert

   Myt1l (MYT1L Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HaloTag-LacI-mHP1a

   Plasmid

   #232637

   Purpose

   lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha

   Depositor

   Karsten Rippe

   Insert

   HaloTag-LacI-mHP1a (Cbx5 Mouse, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1+ dgat1a-FLAG

   Plasmid

   #232143

   Purpose

   Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1a (dgat1a Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. dSpyCas9-E566-GS-LOV2-VPR

   Plasmid

   #231576

   Purpose

   Potent light-deactivated CRISPRa

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   dSpyCas9-VPR with an AsLOV2 insertion behind E566
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PAC_E83_LOV_eGFP

   Plasmid

   #231574

   Purpose

   This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Kif1A-EGFP-SNAP

   Plasmid

   #229851

   Purpose

   Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif1A-EGFP-SNAP (Kif1a Rat)
   

   Use

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SOPP3-HA-nes

    Plasmid

    #229071

    Purpose

    Expresses SOPP3 in the cytosol of mammalian cells

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express SOPP3 in the cytosol of mammalian cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRII-TOPO-dgat1b in situ probe

    Plasmid

    #232138

    Purpose

    zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1b
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRII-TOPO-mogat2 in situ probe

    Plasmid

    #232139

    Purpose

    zebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat2 (mogat2 Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Galt1-HT7-mGold

    Plasmid

    #230027

    Purpose

    Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-HaloTag7-mGold (B4GALT1 Human, Synthetic)
    

    Use

    Tags

    HaloTag,mGold

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid

    #222016

    Purpose

    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO2 (PHOSPHO2 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    chicken β-actin promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid

    #222017

    Purpose

    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO2 (PHOSPHO2 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    deleted stop codon (TAA)

    Promoter

    lac promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AICSDP-167:CDH5-mEGFP

    Plasmid

    #217425

    Purpose

    Homology arms and mEGFP sequence for C-terminus tagging of human cadherin 5

    Depositor

    Allen Institute for Cell Science

    Insert

    CDH5 Homology Arms with mEGFP (CDH5 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP

    Expression

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

    Plasmid

    #225442

    Purpose

    BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

    Depositor

    Diego Orzaez

    Insert

    LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

    Plasmid

    #225441

    Purpose

    Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

    Depositor

    Diego Orzaez

    Insert

    hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSN667

    Plasmid

    #219963

    Purpose

    Expressed sfGFP fused human KIF1Bß(1-721) in insect cells

    Depositor

    Shinsuke Niwa

    Insert

    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    

    Use

    Tags

    sfGFP::2xStrepII tag

    Expression

    Insect

    Mutation

    encodes 1-721

    Promoter

    polyhedrin

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MARS-nGFP

    Plasmid

    #205234

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA3-2xMARS-PLD

    Plasmid

    #205237

    Purpose

    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

    Depositor

    Jeremy Baskin

    Inserts

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    

    Use

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSN668

    Plasmid

    #219962

    Purpose

    Expressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cells

    Depositor

    Shinsuke Niwa

    Insert

    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    

    Use

    Tags

    sfGFP::2xStrepII tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald

    Plasmid

    #214469

    Purpose

    Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TCF4 - P381L in pENTR

    Plasmid

    #216570

    Purpose

    gateway cloning donor vector containing TCF4 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P381L

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TCF4 - D424N in pENTR

    Plasmid

    #216571

    Purpose

    gateway cloning donor vector containing TCF4 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    D424N

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAG426-GAL-APP-CTF

    Plasmid

    #181732

    Purpose

    Galactose inducible expression of APP-CTF

    Depositor

    Alejandro Chavez

    Insert

    APP-CTF (APP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C-terminal fragment (aa 671-770)

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PE-SUMO CRYGS Q16C

    Plasmid

    #217674

    Purpose

    For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6XHis and SUMO

    Expression

    Bacterial

    Mutation

    Glutamine 16 changed to Cysteine

    Promoter

    T7

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid

    #219653

    Purpose

    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

    Depositor

    Thomas Oertner

    Insert

    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    

    Use

    AAV

    Tags

    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

    Expression

    Mammalian

    Mutation

    Promoter

    nEf Addgene # 149296

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28-H12TEV-hEIF5B-587~1220

    Plasmid

    #208637

    Purpose

    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MECP2 - S411R in pENTR

    Plasmid

    #216603

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    S411R

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MECP2 - P388S in pENTR

    Plasmid

    #216602

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P388S

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MECP2 - P376S in pENTR

    Plasmid

    #216601

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P376S

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MECP2 - E282G in pENTR

    Plasmid

    #216600

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    E282G

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MECP2 - P217L in pENTR

    Plasmid

    #216599

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P217L

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MECP2 - A202G in pENTR

    Plasmid

    #216598

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    A202G

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MECP2 - G195S in pENTR

    Plasmid

    #216597

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    G195S

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MECP2 - P152L in pENTR

    Plasmid

    #216596

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P152L

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MECP2 - P152R in pENTR

    Plasmid

    #216595

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    P152R

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MECP2 - R91W in pENTR

    Plasmid

    #216593

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    R91W

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21b-hHNF4aORF-1

    Plasmid

    #206959

    Purpose

    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TRE-tdTomato

    Plasmid

    #210512

    Purpose

    tdTomato reporter vector for LAUNCHER system

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid

    #208979

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid

    #208626

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 191~307, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid

    #208627

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 299~398, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid

    #208629

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 212~285, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid

    #208647

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCW-LIC-N8C_Fab5a

    Plasmid

    #203693

    Purpose

    Expresses N8C_Fab5a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab5a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCW-LIC-N8C_Fab2a

    Plasmid

    #203688

    Purpose

    Expresses N8C_Fab2a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCW-LIC-N8C_Fab1b

    Plasmid

    #203691

    Purpose

    Expresses N8C_Fab1b

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab1b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202024

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GvpA T6A-L40A

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge