We narrowed to 9,825 results for: 158

1. pGEX5X.1 PINK1 KD

   Plasmid

   #13322

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 kinase domain (PINK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acids 112-496; Kinase dead: K219D/D362A/D3…

   Promoter

   Available since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-ACBEmax

   Plasmid

   #139106

   Purpose

   Mammalian ACBEmax expression vector (pSI-632)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ABE8.17-d

   Plasmid

   #136299

   Purpose

   expresses ABE8.17-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2-FLAG-hEcto/Tif1g-CAmut

   Plasmid

   #20903

   Depositor

   Stefano Piccolo

   Article

   Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

   Insert

   Ectodermin (TRIM33 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Point mutagenesis of C125 and C128 to Alanine (RI…

   Promoter

   Available since

   May 15, 2009

   Availability

   Academic Institutions and Nonprofits only

5. anxA10-GFP

   Plasmid

   #107199

   Purpose

   Expresses human annexin A10-GFP fusion protein for live cell imaging

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

   Insert

   annexin A10 (ANXA10 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NC3 pCDNA3.1 FLAG WTX (1-804)

   Plasmid

   #36958

   Depositor

   Randall Moon

   Article

   Major et al Science. 2007 May 18;316(5827):1043-6.

   Insert

   WTX (AMER1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pSpCas9-Puro HLAG-2A-sgRNA

   Plasmid

   #182551

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2A-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. plenti-UbcP-Myc-CRBN-pGK-HYG

   Plasmid

   #107376

   Purpose

   Lentiviral expression of myc-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   UbcP

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFudioFRTPSD-95TealW

   Plasmid

   #134299

   Purpose

   Expresses PSD95 fused to Teal fluorescent protein upon coexpression with the recombinase flippase (Flp)

   Depositor

   Elly Nedivi

   Article

   Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

   Insert

   PSD-95-Teal (Dlg4 Rat, Mouse)
   

   Use

   Lentiviral; Flp/frt

   Tags

   Teal

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-Flag-LmoCas6

    Plasmid

    #126485

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-Flag-LmoCas8b2

    Plasmid

    #126482

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-Flag-LmoCas7

    Plasmid

    #126483

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-Flag-LmoCas5

    Plasmid

    #126484

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. plenti-UbcP-HA-YWAA-pGK-HYG

    Plasmid

    #107379

    Purpose

    Lentiviral expression of HA-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pv9_CBX7_2xChromo-13XL-BASU

    Plasmid

    #179413

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7.2xChromo-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid

    #181844

    Purpose

    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-AKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV_UdgX-EE-UdgX-nCas9-RBMX

    Plasmid

    #163565

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-RBMX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO DNAJB6b

    Plasmid

    #19474

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB6b (DNAJB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCWXPGR-pTF-Wnt3a

    Plasmid

    #114287

    Purpose

    TET-inducible expression of Wnt3a

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM5-mDPYD

    Plasmid

    #61614

    Purpose

    The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    dihydropyrimidine dehydrogenase (Dpyd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge