We narrowed to 12,214 results for: LEA
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Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
Tags6X Histidine Tag, TEV cleavage siteExpressionBacterialPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9
Plasmid#163561PurposeMammalian CG-to-GC base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUdgX-APOBEC1-UdgX-HF-NG-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…Available sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RsSHC014-RBD-8h
Plasmid#161826PurposeMammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014DepositorInsertReceptor-binding domain (RBD) of spike protein S
TagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T321-D519)PromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEP4-BG505.SOSIP-gp160
Plasmid#123273PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHIV-1 (BG505) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutationsPromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 FS1
Plasmid#113955Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, one base pair dele…PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-GRM3-VN
Plasmid#98965PurposeFLAG-tagged human mGluR3 fused to N-terminus of split VenusDepositorInsertGRM3 (GRM3 Human)
TagsFLAG (dykddddk) epitope tag, Signal/leader sequen…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-hAR-CA576-Z
Plasmid#89108Purposemammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthAR-C576A (AR Human)
ExpressionMammalianMutationC576APromoterCMVAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMIG-hH4-AVITEV
Plasmid#74051Purposeretroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman Histone-H4 (H4C16 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianPromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75237PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) FLAG-Ago2 S387E
Plasmid#60653PurposeExpresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
TagsCMV-FLAG-Ago2 (N-term tag)ExpressionMammalianMutationS387E mutantPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) FLAG-Ago2 S387A
Plasmid#60652PurposeExpresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cellsDepositorAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Swachmann-Bodian-Diamond Syndrome Gene, sbds_R (OZ546)
Plasmid#28071DepositorInsertZinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds (sbds Zebrafish)
UseZebrafish targetingAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 long-mut-let-7
Plasmid#21642DepositorPublicationInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant let-7 microRNA binding sitesAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta 610-615
Plasmid#19941DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcullin 1 delta 610-615 (CUL1 Human)
TagsHAExpressionMammalianMutationCUL1 with a ROC1 binding mutantAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-eTLR
Plasmid#214737Purposeencodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAAVS1-eTLR (PPP1R12C Human, Synthetic, Bovine, Rabbit, Chicken, CMV, Anguilla japonica, influenza)
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-SOX2-p2a-KLF4
Plasmid#193292Purposeepisomal hSK (expressing human SOX2 + KLF4)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationF2A of original pCXLE-SK vector was replaced with…PromoterCAGAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q
Plasmid#184249PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
Tags6x His-Tag and TEV cleavage siteExpressionBacterialMutationCodon optimization for protein expression in BL2…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2XSmBiT-SARS-CoV-2 S1
Plasmid#173430Purposeprotein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 FcDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
UseLuciferaseTagshuman IgG1 FcExpressionMammalianMutationfurin cleavage site RRAR to GSASPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046942
Plasmid#145757PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pelB-MBP-BB0238-ss
Plasmid#137046PurposeE. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66635.2 (BB_0238 Borrelia burgdorferi B31)
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0238: lacks the BB0238 signal peptidePromoterT7lacAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only