We narrowed to 18,452 results for: Met

1. pmScarlet_H2A_C1

   Plasmid

   #85051

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   H2A (H2AC16 Human)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-3c-rhVTN-NC

   Plasmid

   #30226

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with N and C terminus cleavages (VTN Human)
   

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478, 20-62

   Available Since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR SFFVp PHAkt Cerulean

   Plasmid

   #50837

   Purpose

   Fluorescent reporter for membrane PIP3 levels

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PHAkt (AKT1 )
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Cerulean

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJC6-HK2-3xFLAG

   Plasmid

   #163451

   Purpose

   lentiviral overexpression vector for human HK2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   HK2 (HK2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   Ubc

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmScarlet-i_H2A_C1

   Plasmid

   #85053

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   H2A (H2AC16 Human)
   

   Tags

   mScarlet-i

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-Blast SLC5A7

   Plasmid

   #218539

   Purpose

   human SLC5A7 cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SLC5A7 (SLC5A7 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-Blast SLC44A1

   Plasmid

   #218538

   Purpose

   human SLC44A1 cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SLC44A1 (SLC44A1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgArid1a/Cre

   Plasmid

   #89642

   Purpose

   Expresses an Arid1a-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   gRNA targeting Arid1a (Arid1a Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and hPGK

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-FLEX-eNpHR-EYFP

    Plasmid

    #89876

    Purpose

    To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    eNpHR=EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmScarlet-i_alphaTubulin_C1

    Plasmid

    #85047

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Alpha-Tubulin (TUBA1B Human)
    

    Tags

    mScarlet-i

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-phiC31-LP-TC

    Plasmid

    #183755

    Purpose

    AAVS1 targeting construct with the phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-AsCpf1-Blast

    Plasmid

    #84750

    Purpose

    Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    AsCpf1
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-3xHA (C terminal on insert)

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. tdTomato-Gamma-Tubulin-17

    Plasmid

    #58099

    Purpose

    Localization: Centrosomes, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET28a-SUMO-MjDim1

    Plasmid

    #189936

    Purpose

    Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

    Depositor

    Chuan He

    Article

    Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

    Insert

    rsmA (MJ_RS00455 Methanocaldococcus jannaschii)
    

    Tags

    6x His tag, FLAG tag, SUMO tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH_Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65267

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTag-BFP-C-h-Rab5a-c-Myc

    Plasmid

    #79801

    Purpose

    Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab5a (RAB5A Human)
    

    Tags

    TagBFP and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Ii-Str_TNF-SBP-EGFP

    Plasmid

    #65280

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pST1374-N-NLS-flag-linker-Cas9-D10A

    Plasmid

    #51130

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    D10A (nickase mutant)

    Promoter

    CMV

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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