Purpose
Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
Inserts
codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
Use
Tags
nuclear export signal (NES)
Expression
Mammalian
Mutation
Y306A/Y384F (AF) double mutant of Methanosarcina …
Available since
May 31, 2022
Availability
Academic Institutions and Nonprofits only