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We narrowed to 56,765 results for: plasmid

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Showing: 3821 - 3840 of 56765 results

  2. SP6-VEE-OKS-iM

    Plasmid
    #58973
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58973-plasmid-map-sequence-id-91416

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  4. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available Since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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  8. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  10. MAPK1

    Plasmid
    #73248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK1 (MAPK1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73248-plasmid-map-sequence-id-137205

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  11. plRL19

    Plasmid
    #163634
    Purpose
    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Insert
    L5 Integrase
    Expression
    Bacterial
    Promoter
    Mycobacterial Optimized Promoter (MOP) and Tet-On
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163634-plasmid-map-sequence-id-339549

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  13. pT3-EF1aH

    Plasmid
    #180149
    Purpose
    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.
    Depositor
    Xin Chen
    Article
    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180149-plasmid-map-sequence-id-357865

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  16. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  20. pPbcas13b

    Plasmid
    #89906
    Purpose
    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89906-plasmid-map-sequence-id-178224

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Showing: 3821 - 3840 of 56765 results