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pJH721_pJH_LldR_PlldR-sfGFP.20TAG

Plasmid

#163729

Purpose

Lactate-inducible expression of green fluorescent protein encoding twenty amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.20TAG

Expression

Bacterial

Mutation

gfp with all 20 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH727_pJH_LldR_PlldR-sfGFP.10TAG

Plasmid

#163731

Purpose

Lactate-inducible expression of green fluorescent protein encoding ten amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH774_pJH_LldR_PlldR-sfGFP.1opal

Plasmid

#163733

Purpose

Lactate-inducible expression of green fluorescent protein encoding one opal codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TGA

Expression

Bacterial

Mutation

gfp with first leucine codon mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 Bighead S-HA

Plasmid

#172485

Purpose

for synthesis of mRNA for injection into xenopus laevis embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

In vitro mrna synthesis

Tags

HA and S-HA

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Tags

HA

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET-11a HIV-1 P6

Plasmid

#154201

Purpose

Bacterial expression of Gag P6 from HIV-1 HXB2 strain

Depositor

Lalit Deshmukh

Article

Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

Insert

Gag P6 protein from HIV-1 clone HXB2

Tags

GB1-6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm3_A

Plasmid

#145888

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-mu68

Plasmid

#162662

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68

Plasmid

#162625

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-mu68

Plasmid

#162627

Purpose

Expresses N-terminal 2x-Strep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL52

Plasmid

#162628

Purpose

Expresses N-terminal 2x-Strep-tagged UL52 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL52

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32

Plasmid

#162629

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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HRP-1LC

Plasmid

#139116

Purpose

express the LC of HRP-1

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Bacterial

Mutation

181-346

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N1

Plasmid

#160988

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N2-2A2

Plasmid

#160991

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N2

Plasmid

#160989

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N1-2A2

Plasmid

#160990

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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p5343_UC57

Plasmid

#126645

Purpose

environmental plasmid p5343 cloned into pUC57

Depositor

Aindrila Mukhopadhyay

Article

Kothari et al mSystems. 2019 Dec 17;4(6). pii: 4/6/e00

Insert

Environmental plasmid p5343

Use

Synthetic Biology

Promoter

NA

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ AMIC

Plasmid

#118420

Purpose

For mammalian expression of Amoeba myosin 1C

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

Amoeba myosin 1C

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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S11_EcoRV_CAMKIIA_AscI

Plasmid

#128475

Purpose

CAMKII1 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

CAMKII1 promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S10_EcoRV_hcSYN_AscI

Plasmid

#128474

Purpose

hcSYN promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

hcSYN promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-TAK1

Plasmid

#117162

Purpose

Target site AGCGCCCTTCAATGGAGGAAAT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-STAT2

Plasmid

#117161

Purpose

Target site TTTAAGTTCCACAGACTTGGA

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pKN3097

Plasmid

#113392

Purpose

Galactose-inducible Ty3 with extra PPT- used for random bar code library

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Ty3

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pT7T3D-PacI-Tmeff2

Plasmid

#99728

Purpose

to make in situ RNA probes for Tmeff2

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Use

In situ probe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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