We narrowed to 4,634 results for: HRE

1. pLenti puro HA-Ubiquitin

   Plasmid

   #74218

   Purpose

   Lentiviral expression of HA tagged Ubiquitin

   Depositor

   Melina Fan

   Insert

   ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available Since

   March 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pTHSSd_14

   Plasmid

   #59952

   Purpose

   Triple split (at 67, 601-SZ)

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   Three-fragment T7* RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PTac

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB4581

   Plasmid

   #171124

   Purpose

   A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

   Insert

   3_sAID
   

   Use

   Other

   Tags

   Expression

   Mutation

   non applicable

   Promoter

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS1106_Tier3(Lenti)

   Plasmid

   #169661

   Purpose

   Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TtTMPV-Neo

   Plasmid

   #27993

   Depositor

   Scott Lowe

   Article

   Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

   Insert

   TtTMPV-Neo (shRen)
   

   Use

   RNAi and Retroviral; Fluorescent reporters

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. CS2 Flag-Smad3 (3A)

   Plasmid

   #14964

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

   Insert

   Smad3 (3A) (SMAD3 Human)
   

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   Flag

   Expression

   Mutation

   Three Ser to Ala mutations in the phosphorylation…

   Promoter

   Available Since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVT-myc-T58A-S62D

   Plasmid

   #138540

   Purpose

   For making myc-T58A-S62D RNA

   Depositor

   Carmen Williams

   Insert

   Myelocytomatosis oncogene T58A S62D (Myc Mouse)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Threonine 58 changed to alanine and serine 62 cha…

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSaI14

   Plasmid

   #8614

   Depositor

   Robert Hartley

   Article

   Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

   Insert

   Ribonuclease inhibitor SaI14
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N terminal threonine changed to alanine

   Promoter

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pSaI20

   Plasmid

   #8615

   Depositor

   Robert Hartley

   Article

   Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

   Insert

   Ribonuclease inhibitor SaI20
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N terminal threnine changed to alanine

   Promoter

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS1108_Tier3(Lenti_inverse)

    Plasmid

    #169663

    Purpose

    Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction in inverted direction with polyA (PCMV-5'LTR-Psi-gag-env-A3-pA::A2-pA::A1-pA-WPRE-3'LTR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACλCI-β-flap (831-1057)

    Plasmid

    #53733

    Purpose

    Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    λCI-β-flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac UV5

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. BMPR2

    Plasmid

    #38972

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BMPR2 (BMPR2 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGFP-Rab8A[T22N]

    Plasmid

    #24899

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A[T22N]
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 22 to Asparagine

    Promoter

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBa-MARK2-eGFP

    Plasmid

    #66706

    Purpose

    mammalian expression vector for MARK2 with a C-terminal eGFP

    Depositor

    Marvin Bentley, Gary Banker

    Insert

    MARK2 (MARK2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta actin

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV Flag ATRwt

    Plasmid

    #41909

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 2001 Nov 23;294(5547):1713-6.

    Insert

    ATR ATR serine/threonine kinase (ATR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SRa

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE30-His-ThrRS

    Plasmid

    #124119

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Threonyl-tRNA ligase (thrS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-puro-shFAM134B

    Plasmid

    #109013

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    shFAM134B (RETREG1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMT-PTE2A

    Plasmid

    #111773

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH628_pJH_LldR_PlldR-sfGFP.3TAG

    Plasmid

    #163727

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding three amber codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.3TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp.N39TAG,N135TAG,Y151TAG

    Promoter

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAC-BA-lacZ

    Plasmid

    #13423

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    BA
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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