We narrowed to 5,898 results for: dna plasmids

1. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

   Plasmid

   #121833

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/Dnmt3a-3L (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-Cas9/CD4-TK2

   Plasmid

   #104397

   Purpose

   The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

   Depositor

   Jeronimo Blanco

   Article

   Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

   Insert

   TK2 (TK2 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

   Plasmid

   #121826

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/Dnmt3a-3L (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

   Plasmid

   #182894

   Purpose

   Mammalian expression of PVALB rat mAb HC gene

   Depositor

   Heng Zhu

   Insert

   anti-PVALB (human) heavy chain (PVALB Rat)
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Mutation

   No

   Promoter

   CMV

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Slack Slo2.2 max1-ECFP

   Plasmid

   #197318

   Purpose

   Plasmid expressing an antigen used to generate an antibody that targets the Slack K+ channel subunit

   Depositor

   Christopher Lingle

   Insert

   KCNT1/Slo2.2/Slack K+ channel (Kcnt1 Mouse)
   

   Tags

   ECFP

   Expression

   Mammalian

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nemato

   Plasmid

   #24006

   Depositor

   Lawrence Cohen

   Insert

   VSFP
   

   Tags

   Cerulean and Citruline

   Expression

   Mammalian

   Available Since

   April 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOME0294M

   Plasmid

   #182623

   Purpose

   KSHV anti-RTA_3Kb_Reverse

   Depositor

   Dirk Dittmer

   Insert

   KSHV anti-RTA_3Kb_Reverse
   

   Expression

   Mammalian

   Mutation

   Deletion at 2853

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Luc-noPS

   Plasmid

   #177940

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-noPS

   Plasmid

   #177943

   Purpose

   Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NanoBRET BiBRET Vector, NanoLuc-KRAS(G12D)_CRAF(RBD-CRD)-HaloTag

    Plasmid

    #238576

    Purpose

    Express NanoLuc-KRAS(G12D)_CRAF(RBD-CRD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    CRAF(RBD-CRD) (RAF1 Human)
    

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. NanoBRET BiBRET Vector, NanoLuc-KRAS WT-CRAF RBD-CRD-HaloTag

    Plasmid

    #236865

    Purpose

    Express NanoLuc(R)-KRAS WT-CRAF RBD-CRD-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD)-CRD (RAF1 Human)
    

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. NanoBRET BiBRET Vector HaloTag-KRAS 2B WT-BRAF RBD-NanoLuc

    Plasmid

    #236853

    Purpose

    Express HaloTag(R)-KRAS 2B WT-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    BRAF (RBD) (BRAF Human)
    

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. L-SACSIN-74

    Plasmid

    #166526

    Purpose

    scFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOME0295M

    Plasmid

    #182624

    Purpose

    KSHV Reverse Sequence Negative control Kaposinclouster_RevCompl-KapATGdel_Syn

    Depositor

    Dirk Dittmer

    Insert

    KSHV Kaposincluster_RevCompl_KapATGdel_SYN_rev
    

    Expression

    Mammalian

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid

    #182895

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid

    #163643

    Purpose

    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    

    Use

    Lentiviral

    Tags

    Luc2-P2A-tdTomato-T2A

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    EFS and pU6

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid

    #74408

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. CDKN1A (p21) targeting gRNA

    Plasmid

    #215319

    Purpose

    Expresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.10 EXOC3prom SV40

    Plasmid

    #246713

    Purpose

    To investigate the influence of a universal enhancer (SV40) on EXOC3 promoter activity (measured by luciferase expression)

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Inserts

    EXOC3 Promoter (EXOC3 Human)
    SV40 universal enhancer
    

    Use

    Luciferase

    Mutation

    WT

    Available Since

    Nov. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4.10 EXOC3prom Chr5-LG4

    Plasmid

    #246712

    Purpose

    To investigate the influence of an LG4 enhancer (chr5 LG4) on EXOC3 promoter activity (measured by luciferase expression)

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Inserts

    EXOC3 Promoter (EXOC3 Human)
    chr5 LG4 enhancer
    

    Use

    Luciferase

    Mutation

    WT

    Available Since

    Nov. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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