We narrowed to 5,985 results for: crispr cas9 expression plasmids

1. OgeuIscB TGC RNA

   Plasmid

   #222863

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a TGC target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (TGC)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. OgeuIscB AGC RNA

   Plasmid

   #222866

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a AGC target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (AGC)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. OgeuIscB CAG RNA

   Plasmid

   #222867

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a CAG target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (CAG)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pRGEB34

   Plasmid

   #158152

   Purpose

   Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

   Plasmid

   #174137

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. U6-(BbsI)sgRNA_CAG-Venus-bpA

   Plasmid

   #86985

   Purpose

   For cloning and expression of sgRNA together with Venus expression

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Venus GFP variant

   Promoter

   CAG

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYC2085

   Plasmid

   #158721

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMLM3705

   Plasmid

   #47754

   Purpose

   Expresses mammalian cell codon-optimized dCas9-VP64

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   codon optimized dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9 (catalytically i…

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PU6::klp-12_sgRNA

    Plasmid

    #46170

    Purpose

    klp-12 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    klp-12 targeting sgRNA (klp-12 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge