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pMC-1-EXOB-11

Plasmid

#197688

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EXOB enhancer-blocking insulator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x6

Plasmid

#58776

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PEP103E

Plasmid

#97389

Purpose

binary construct of plastid-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Plastid target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PEP105E

Plasmid

#97391

Purpose

binary construct of peroxisome-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Peroxisome target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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CeNL-Actin_pcDNA3

Plasmid

#89512

Purpose

Cyan color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z17

Plasmid

#120060

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P14

Plasmid

#120025

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z09

Plasmid

#120052

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P15

Plasmid

#120026

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_600/pcDNA3

Plasmid

#51982

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-2G

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pTRF.1-mCMV-udsVenus

Plasmid

#58518

Purpose

Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MTUS1 zeo

Plasmid

#192323

Purpose

Lentiviral expression vector for an inducible 3xFLAG-MTUS1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

MTUS1 (MTUS1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P24

Plasmid

#120035

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P18

Plasmid

#120029

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_250/pcDNA3

Plasmid

#85206

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_250

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P10

Plasmid

#120021

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GL2 shRNA

Plasmid

#86083

Purpose

A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

control shRNA

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_1.2μ-pcDNA3

Plasmid

#111926

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_1.2μ

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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7iBi89 (rat fibronectin)

Plasmid

#14065

Depositor

Richard Hynes

Article

Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

Insert

Rat Fibronectin 7iBi89 (Fn1 Rat)

Expression

Mammalian

Mutation

Insert was cut out of pGEM3 with SalI and SacI an…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pDREV-0

Plasmid

#26750

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

H2B-Venus CDS (H2BC3 Human)

Use

Cre/Lox; Flp/frt; dre/rox

Tags

Venus

Expression

Mammalian

Available Since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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