159,557 results

1. pBG-ttLbUV2

   Plasmid

   #231386

   Purpose

   LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

   Depositor

   Qi-Jun Chen

   Article

   Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

   Insert

   ttLbCas12a Ultra V2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D156R and E795L

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL hygro Flag HA TRAP220 wt

   Plasmid

   #17433

   Purpose

   Retroviral expression of Med1/TRAP220 in mammalian cells

   Depositor

   Kai Ge

   Article

   Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

   Insert

   TRAP220 (MED1 Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIPZ-EGFP:Ajuba

   Plasmid

   #108229

   Purpose

   Lentiviral vector for Tet-inducible Ajuba expression

   Depositor

   Kenneth Irvine

   Article

   Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

   Insert

   AJUBA (AJUBA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tet/ON Minimal CMV promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CS2 Flag-Smad2

   Plasmid

   #14042

   Depositor

   Joan Massague

   Article

   Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

   Insert

   Smad2 (SMAD2 Human)
   

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-LIC

   Plasmid

   #62303

   Purpose

   E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

   Plasmid

   #50463

   Purpose

   Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. eGFP-proα2(I)

   Plasmid

   #119826

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rich-pT7GG

   Plasmid

   #108443

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Xenopus oocyte expression system

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet-1 TIM9,10

   Plasmid

   #170280

   Purpose

   Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

   Insert

   mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. K44A HA-dynamin 1 pcDNA3.1

    Plasmid

    #34683

    Depositor

    Sandra Schmid

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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