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pET51b-His-TEV-CLIP-tag

Plasmid

#167274

Purpose

Overexpression of CLIP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV_3C-Twin-Strep

Plasmid

#113900

Purpose

Mammalian protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

SFFV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 Peroxisome visual barcode

Plasmid

#158668

Purpose

tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrispr-V2-mOrange (V2mO)

Plasmid

#140206

Purpose

Lenti Crispr/Cas9 with mOrange fluorescent marker

Depositor

Jaffer Ajani

Article

Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

pELF1a

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

Plasmid

#61594

Purpose

A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D10A, N580A

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-Hph

Plasmid

#188741

Purpose

pUC19 plasmid (MCS intact) with Hygromycin selection marker

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

Hygromycin

Use

Filamentous fungal expression

Promoter

gpdA

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pOPINB-AMSH*

Plasmid

#66712

Purpose

Expresses activated AMSH in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

hbp (Stam2 Mouse)
AMSH (STAMBP Human)

Tags

His-3C

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pET51b-His-TEV-SNAP-tag

Plasmid

#167269

Purpose

Overexpression of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (WT)

Plasmid

#169720

Purpose

Mammalian Expression of wild-type mouse SPOP

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-P2A-APEX2-VHH[GFP]

Plasmid

#126749

Purpose

Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

VHH[EGFP]

Use

Zebrafish muscle expression clone

Tags

mKate2-P2A-APEX2

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7FTrp)RS

Plasmid

#177310

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-FUSN

Plasmid

#200967

Purpose

induction of phase-separated condensate mediated by FUSN-IDR

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

FUSN

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep

Plasmid

#113883

Purpose

Mammalian (inducible) protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

Plasmid

#62897

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
actc1b-2PH-PLCd-mKate2

Plasmid

#109500

Purpose

Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-2PH-PLCd-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAT-WT-Myc

Plasmid

#177717

Purpose

ER protein alpha-1-antitrypsin (WT), myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

Myc

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

Plasmid

#20943

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only

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TVMV-TEV-HRV-3C Proteases-mCherry

Plasmid

#243798

Purpose

Encodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TVMV, TEV and HRV-3C proteases

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP Peroxisome visual barcode

Plasmid

#158677

Purpose

tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

EYFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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