We narrowed to 24,606 results for: Nes

1. pMC-6-GFP-P2A-NptII-9

   Plasmid

   #197741

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   EGFP-P2A-NptII bicistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-vioABECD

   Plasmid

   #66535

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Consensus T7

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHL-FcHis

   Plasmid

   #99846

   Purpose

   mammalian expression with secretive signal and FC tag

   Depositor

   Edith Yvonne Jones

   Article

   Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   ephrin b2 (EPHB2 Human)
   

   Use

   Tags

   Fc_His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. YE2-BE4max

   Plasmid

   #138156

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YE2)-nSpCas9-UGI-UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGB 35S:Renilla:Tnos (GB0109)

   Plasmid

   #68215

   Purpose

   Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Renilla
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2/CMV-Luc/PGK-SB100X

   Plasmid

   #192869

   Purpose

   Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

   Depositor

   Maria Castro, David Largaespada

   Article

   Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

   Inserts

   luc
   SB100X
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Zebrabow-GateDest

   Plasmid

   #118221

   Purpose

   For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

   Depositor

   Alex Schier, Albert Pan

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L - ccdB survival cassette
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Thy1 promoter construct

   Plasmid

   #20736

   Depositor

   Joshua Sanes

   Article

   Feng et al Neuron. 2000 Oct . 28(1):41-51.

   Insert

   Thy1 promoter (Thy1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. TB:5xNF- κβ_RE:MiniP

   Plasmid

   #133878

   Purpose

   5 copies of the NF-κβ DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   5 copies of the NF-kb DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TB:7xE-Box:MiniP

    Plasmid

    #133880

    Purpose

    7 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies of the E-box motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC-6-BeYDV-Rep-RepA-9

    Plasmid

    #197715

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV Rep/RepA proteins: native ORFs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 shSV40 neo

    Plasmid

    #158646

    Purpose

    Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    SV40Tag (SV40gp6 Simian virus 40)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetroX-Tight-MCS_PGK-GpNLuc

    Plasmid

    #70185

    Purpose

    Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRetroX-Tight-MCS_PGK-OgNLuc

    Plasmid

    #70186

    Purpose

    Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGB 35S:YFP:Tnos (GB0209)

    Plasmid

    #68219

    Purpose

    Transcriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCerulean3-Rap1

    Plasmid

    #134928

    Purpose

    Fluorescently tagged GTPase.

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1B, Cerulean
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-FLEX-SaCas9-U6-sgSlc17a8

    Plasmid

    #124848

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge