We narrowed to 8,593 results for: Set

1. N-Terminal Split Cas9 D10A Nickase with GyrA intein

   Plasmid

   #58695

   Purpose

   Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
   

   Use

   AAV and CRISPR

   Tags

   GyrA Nsplit Intein, HA Tag, and NLS

   Expression

   Mammalian

   Mutation

   D10A nickase converting mutation to SpCas9

   Promoter

   CBh

   Available Since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-eGFP-SHLD3

   Plasmid

   #114126

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD3 (SHLD3 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. PSD-95 scFv [K28/43]

   Plasmid

   #182066

   Purpose

   Mammalian Expression of PSD-95 scFv. Derived from hybridoma K28/43.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting PSD-95 (Homo sapiens) (DLG4 Mouse)
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. NPY/Neuropeptide Y scFv [L115/13]

   Plasmid

   #190505

   Purpose

   Mammalian Expression of NPY/Neuropeptide Y scFV. Derived from hybridoma L115/13.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   NPY/Neuropeptide Y (Homo sapiens) recombinant scFV (NPY Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Homer1L scFv [L113/13]

   Plasmid

   #190498

   Purpose

   Mammalian Expression of Homer1L scFV. Derived from hybridoma L113/13.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Homer1L (Mus musculus) recombinant scFV (Homer1 Mouse)
   

   Tags

   Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-LAR/PTPRF [N165/38R]

   Plasmid

   #177504

   Purpose

   Mammalian Expression Plasmid of anti-LAR/PTPRF (Human). Derived from hybridoma N165/38.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-LAR/PTPRF (Homo sapiens) recombinant mouse monoclonal antibody (PTPRF Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-Pan-Neurofascin (extracellular) [A12/18R]

   Plasmid

   #177438

   Purpose

   Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-ATF4 [N360A/24]

   Plasmid

   #190315

   Purpose

   Mammalian Expression Plasmid of anti-ATF4 (Human). Derived from hybridoma N360A/24.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-ATF4 (Homo sapiens) recombinant Mouse monoclonal antibody (ATF4 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-46: MYL7-mEGFP

   Plasmid

   #114413

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-Pan-PTPR [N165/43R]

    Plasmid

    #188219

    Purpose

    Mammalian Expression Plasmid of anti-Pan-PTPR. Derived from hybridoma N165/43

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Pan-PTPR recombinant mouse monoclonal antibody (PTPRF Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Anti-IRSp53/BAIAP2 [L117/1R]

    Plasmid

    #177462

    Purpose

    Mammalian Expression Plasmid of anti-IRSp53/BAIAP2 (Human). Derived from hybridoma L117/1.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-IRSp53/BAIAP2 (Homo sapiens) recombinant mouse monoclonal antibody (BAIAP2 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PSD-95 scFv [K28/74]

    Plasmid

    #190490

    Purpose

    Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/74.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

    Plasmid

    #211969

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

    Plasmid

    #211968

    Purpose

    Express gRNA against LIN28A with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting LIN28A (LIN28A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Anti-SPHKAP [L131/17R]

    Plasmid

    #177477

    Purpose

    Mammalian Expression Plasmid of anti-SPHKAP (Mouse). Derived from hybridoma L131/17.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-SPHKAP (Mus musculus) recombinant mouse monoclonal antibody (Sphkap Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid

    #207355

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-eGFP-SHLD3

    Plasmid

    #114125

    Purpose

    Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    Aug. 16, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Anti-TrpM7 [N74/25R]

    Plasmid

    #177569

    Purpose

    Mammalian Expression Plasmid of anti-TrpM7 (Mouse). Derived from hybridoma N74/25.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-TrpM7 (Mus musculus) recombinant mouse monoclonal antibody (Trpm7 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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