We narrowed to 7,454 results for: ski

1. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

   Plasmid

   #138145

   Purpose

   Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVeBtA1

   Plasmid

   #238986

   Purpose

   Mammalian expression of bovine arrestin-1 with Venus N-terminal fusion

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

   Insert

   arrestin-1 (SAG Bovine)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

   Plasmid

   #194685

   Purpose

   CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_09-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-C2-SNAP

   Plasmid

   #208769

   Purpose

   To express C2-SNAP in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2-SNAP (Mfge8 Mouse)
   

   Use

   Tags

   SNAP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT/TO_TOMM20_HaloTag9

   Plasmid

   #169335

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194687

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pp-mCAT1

   Plasmid

   #83948

   Purpose

   This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

   Depositor

   Vincent Lotteau, Philippe Mangeot

   Article

   Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

   Insert

   mCAT1 (Slc7a1 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MET7A-CD4d3+4-bio

   Plasmid

   #73127

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding MET7A with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   MET7A (METTL7A Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET-pr-Kaede (Kaede-A69T)

   Plasmid

   #99228

   Purpose

   Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-Kaede
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A69T for primed conversion applications

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. W118-1_hTGM3-Flag

    Plasmid

    #180408

    Purpose

    lentiviral transduction of human TGM3 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM3 (TGM3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJL1-SpCas9

    Plasmid

    #117051

    Purpose

    In vitro expression of S. pyogenes Cas9 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    S. pyogenes Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1P shSOD1

    Plasmid

    #102975

    Purpose

    Suppression of SOD1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shSOD1 (SOD1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEY43

    Plasmid

    #191047

    Purpose

    eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    eat-4(prom6-1) (eat-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRY2(L348F)-CreN

    Plasmid

    #75368

    Purpose

    Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Cre recombinase, residues 19-104

    Expression

    Mammalian

    Mutation

    L348F

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET51b-His-TEV-CLIP-tag_fast

    Plasmid

    #167275

    Purpose

    Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SIN40C.SFFV.dTomato.let-7c

    Plasmid

    #169301

    Purpose

    Constitutive overexpression of let-7c with dTomato as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    let-7c (MIRLET7C Human)
    

    Use

    Lentiviral

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEND-400_Cacnes_CAMP2

    Plasmid

    #225606

    Purpose

    Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. W118-1_hTGM4-Flag

    Plasmid

    #180409

    Purpose

    lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM4 (TGM4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. W118-1_hTGM5-V5

    Plasmid

    #180414

    Purpose

    lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM5 (TGM5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-EF1a-IRES-Puro GFP-3xFLAG

    Plasmid

    #201636

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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