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ORF18(R180A)-3xFLAG pCDNA4

Plasmid

#129806

Purpose

Expresses KSHV ORF18 (R180A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 180 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G214A)-3xFLAG pCDNA4

Plasmid

#129808

Purpose

Expresses KSHV ORF18 (G214A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 214 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18 (Y85A)-3xFLAG pCDNA4

Plasmid

#129786

Purpose

Expresses KSHV ORF18 (Y85A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Tyrosine 85 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(R74A_K75A)-3xFLAG pCDNA4

Plasmid

#129811

Purpose

Expresses KSHV ORF18 (R74A_K75A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 74 to Alanine and Lysine 75 to A…

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(W170A_G171A)-3xFLAG pCDNA4

Plasmid

#129815

Purpose

Expresses KSHV ORF18 (W170A_G171A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Tryptophan 170 to Alanine and Glycine 171…

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(R158A)-3xFLAG pCDNA4

Plasmid

#129801

Purpose

Expresses KSHV ORF18 (R158A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 158 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G145A)-3xFLAG pCDNA4

Plasmid

#129796

Purpose

Expresses KSHV ORF18 (G145A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 145 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(N146A)-3xFLAG pCDNA4

Plasmid

#129798

Purpose

Expresses KSHV ORF18 (N146A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Asparagine 146 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG

Plasmid

#127305

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AGG-nLuc-3XFLAG

Plasmid

#127304

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG

Plasmid

#127303

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_Trf2

Plasmid

#125159

Purpose

Gateway entry clone

Depositor

Julius Brennecke, Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

Trf2 (Trf2 Fly)

Use

Gateway Cloning

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET21d_SNORD78_probe

Plasmid

#73072

Purpose

antisense probe for detection of human SNORD78 (C/D box snoRNA U78)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD78 (SNORD78 Human)

Expression

Bacterial

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pUC 3GLA

Plasmid

#71762

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMV-TRP1

Plasmid

#65335

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TRP1

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa V62T

Plasmid

#47630

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Expression

Bacterial

Mutation

Changed Valine 62 to Threonine

Promoter

T7

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pPK1E-2

Plasmid

#48146

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPK1E-1

Plasmid

#48145

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa T147V

Plasmid

#47628

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Expression

Bacterial

Mutation

Changed Theronine 147 to Valine

Promoter

T7

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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Danio rerio cryaa C143S

Plasmid

#47619

Purpose

Recombinant protein production

Depositor

Mason Posner

Article

Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

Insert

Alpha A-crystallin (cryaa Zebrafish)

Expression

Bacterial

Mutation

Cysteine 143 to Serine

Promoter

T7

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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