We narrowed to 5,990 results for: crispr cas9 expression plasmids

1. pHEE401

   Plasmid

   #71286

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   sgRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXPR_050

   Plasmid

   #96925

   Purpose

   lentiviral expression of gRNA scaffold

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFD115

   Plasmid

   #124768

   Purpose

   pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   cas9
   term PpflB sgRNA (BsaI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ptet

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYC1446

   Plasmid

   #158720

   Purpose

   Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. rtTA-sg-mGreenLantern332

   Plasmid

   #188483

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern sgRNA
   

   Use

   CRISPR

   Tags

   PB_rtTA_BsmBI

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. rtTA-sg-CMV152

   Plasmid

   #188485

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. rtTA-sg-tdTomato166/892

   Plasmid

   #188488

   Purpose

   gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. rtTA-sg-tdTomato90/816

   Plasmid

   #188487

   Purpose

   gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. rtTA-sg-CMV162

   Plasmid

   #188486

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. rtTA-sg-CMV51

    Plasmid

    #188484

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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