We narrowed to 5,494 results for: SPI

1. PSUC2-yEGFPCLN2PEST-pRS406

   Plasmid

   #64405

   Purpose

   SUC2 promoter driven GFP expression

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   SUC2 promoter (SUC2 Budding Yeast)
   

   Use

   Tags

   yEGFPCLN2PEST (destabilized yeast EGFP)

   Expression

   Yeast

   Mutation

   Promoter

   SUC2

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJKW1813

   Plasmid

   #174003

   Purpose

   Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

   Depositor

   Jing-Ke Weng

   Article

   Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

   Inserts

   PpCYP90G4
   ATR1 (ATR1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEYFP-Pds1Δdestruction box(383)

   Plasmid

   #39848

   Depositor

   Jonathon Pines

   Article

   Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

   Insert

   Pds1 (PTTG1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Δdestruction box = ΔR61-N68

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-mCherry2-sgC9cen

   Plasmid

   #198326

   Purpose

   Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   Chr9-CEN guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTK158_mCherry-4.1G-deltaCTD-hardened

   Plasmid

   #46360

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   4.1G (EPB41L2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Deleted amino acids 886-1005, and modified for si…

   Promoter

   CMV

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP_C1 I3

   Plasmid

   #61397

   Purpose

   Expresses GFP-tagged Inhibitor-3 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   Inhibitor-3 (PPP1R11 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pIW086

   Plasmid

   #134132

   Purpose

   Bacterial expression of His-TEV-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-TEV-human Ska1 ; human Ska2
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   K183A K184A

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVenus_Lys-less-Cdc20 (1620)

   Plasmid

   #39849

   Depositor

   Jonathon Pines

   Article

   Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

   Insert

   Cdc20 (CDC20 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Lys-less = all Lys converted to Arg

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pNIR-Fb6m

   Plasmid

   #184680

   Purpose

   NIR-Fb to spike-Cov-19 RBD

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fb6m (S Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-MIS12-LAP-BUB1

    Plasmid

    #115904

    Purpose

    Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

    Insert

    MIS12-BUB1 (MIS12 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    BUB1 fused to MIS12

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRII-Topo S100a10 in situ probe

    Plasmid

    #45603

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    s100a10 in situ probe (S100a10 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 21-275 of BC025044

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

12. Venus-Cdc20 R132A (1768)

    Plasmid

    #26059

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (CDC20 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Mutation in the Mad2 binding site, R132A

    Promoter

    Available since

    Aug. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

13. 1kb Zfp521 promoter reporter

    Plasmid

    #104188

    Purpose

    luciferase reporter to assay Zfp521 promoter activation

    Depositor

    Kathryn Hentges

    Article

    Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

    Insert

    Zfp521 promoter - 1 Kb (Zfp521 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTK157_mCherry-4.1G-CTD

    Plasmid

    #46359

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G C-terminal domain (EPB41L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C-terminal domain from 886-1005 amino acids

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBW-m4046938

    Plasmid

    #145599

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCRII-Topo Foxp2 in situ probe

    Plasmid

    #45620

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Foxp2 in situ probe (Foxp2 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp#1709-2142 of AF339106

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

    Plasmid

    #175017

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    OK-HEFwtED
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGN33

    Plasmid

    #131105

    Purpose

    An integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutants

    Depositor

    Riccardo Manganelli

    Article

    Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

    Insert

    P-furA102tetO-pip
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    the furA102 promoter was mutated with the inserti…

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEM01

    Plasmid

    #135414

    Purpose

    Expression of Hygromycin resistance from CMV promoter

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    CMVpr hph ADH1t
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CMVpr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRII-Topo Cux2 in situ probe

    Plasmid

    #45623

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Cux2 in situ probe (Cux2 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains nt 1069-1694 on NM_007804

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only