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pmalc4x_V426F_TEV

Plasmid

#47702

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with V426F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed V426F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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IRS1-A4

Plasmid

#25697

Depositor

Eric Fearon

Article

Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

Insert

IRS1 (IRS1 Human)

Use

RNAi and Retroviral

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pT7-gRNA_zebrafish_mmp21

Plasmid

#72890

Purpose

plasmid to generate guideRNA against mmp21 gene in zebrafish

Depositor

Nicholas Katsanis

Article

Perles et al J Med Genet. 2015 Dec;52(12):840-7. doi:

Insert

zebrafish mmp21 (mmp21 Zebrafish)

Use

CRISPR

Promoter

T7 RNA pol

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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mTFP1-H4-23

Plasmid

#55491

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H4 (HIST1H4J Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Alpha-Actinin-19

Plasmid

#55464

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
helicase cDNA

Plasmid

#39552

Depositor

Norbert Perrimon

Article

Eberl et al Genetics. 1997 Jul;146(3):951-63.

Insert

helicase (Hel25E Fly)

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
pBA1751

Plasmid

#246066

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 ori

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Panoptes locus

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi-MTS-FAP-GFP

Plasmid

#242990

Purpose

Expresses MTS-FAP-GFP in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

MTS-dH6.2-GFP

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi-FAP-GFP-KDEL

Plasmid

#242991

Purpose

Expresses FAP-GFP-KDEL in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-GFP-KDEL

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHD Btl-pYtag(Scarlet) ScarlessDsRed

Plasmid

#244937

Purpose

Vector for tagging Breathless (Btl) with Scarlet-based pYtag biosensor. DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)

Use

Endogenous tagging

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1729

Plasmid

#246063

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Panoptes locus

Expression

Bacterial

Promoter

pTet promoter

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R4A,R5A)

Plasmid

#245221

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

R4A,R5A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Torso

Plasmid

#244933

Purpose

CRISPR gRNA targeting the C-terminal end of Torso for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Torso C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Btl

Plasmid

#244934

Purpose

CRISPR gRNA targeting the C-terminal end of Breathless for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Btl C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-EGFR

Plasmid

#244932

Purpose

CRISPR gRNA targeting the C-terminal end of EGFR for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting EGFR C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Px-PhiYFP-triplex-28-M13-28-pA

Plasmid

#202047

Purpose

Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, Csy4 28nt sites, and cloning site for gRNA to activate downstream targets.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

PhiYFP

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH

Plasmid

#239740

Purpose

Plasmid expressing a rapamycin-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmF

Plasmid

#226912

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmR

Plasmid

#226913

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304 mNG PH PLCD1

Plasmid

#233254

Purpose

Expression of mNeonGreen-tagged PH domain of PLCD1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

PLCD1

Use

Lentiviral

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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