We narrowed to 11,993 results for: pcdna

1. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Cerulean-Epac2-Venus

   Plasmid

   #138196

   Purpose

   FRET-based biosensor for monitoring Epac2 activation.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-HIF2alpha-P405A/P531A-pcDNA3

   Plasmid

   #18956

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Promoter

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1227 pcDNA3 Myr HA Akt2

   Plasmid

   #9016

   Depositor

   William Sellers

   Insert

   Akt2 (AKT2 Human)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)Flag-His-ATM wt

   Plasmid

   #31985

   Purpose

   Mammalian expression of human ATM with Flag and His tags

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM wild-type (ATM Human)
   

   Use

   Tags

   Flag and HIS

   Expression

   Mammalian

   Mutation

   3' UTR of 400 nucl.

   Promoter

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-SARS-CoV-2-S (Wuhan)

   Plasmid

   #215812

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Ndi-1/myc-His

   Plasmid

   #127503

   Purpose

   Expresses myc-tagged yeast Ndi-1 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   Ndi-1 (NDI1 Budding Yeast)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 EYFP H148Q/I152L

   Plasmid

   #25872

   Depositor

   Peter Haggie

   Article

   Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

   Insert

   EYFP H148Q/I152L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP from pEYFP (Clontech) with H148Q and I152L m…

   Promoter

   Available Since

   Aug. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Clover-Prx2-mRuby2

    Plasmid

    #117906

    Purpose

    fluorescent reporter of cytosolic hydrogen peroxide

    Depositor

    Hadley Sikes

    Article

    Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

    Insert

    Clover-Prx2-mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-10His-mStayGold (E138D)

    Plasmid

    #211363

    Purpose

    Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Mammalian

    Mutation

    E138D

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT/TO_TOM20-HaloTag7_T2A_mEGFP

    Plasmid

    #187072

    Purpose

    Mitochondria surface expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

    Insert

    TOM20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid

    #140973

    Purpose

    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai1-RLuc8 (GNAI1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FlipCherry-T2A-GFP

    Plasmid

    #124436

    Purpose

    Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipCherry-T2A-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid

    #236007

    Purpose

    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    D835Y substitution

    Promoter

    CMV

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-DEST47 PINK1 C-GFP

    Plasmid

    #13316

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA flag PPAR gamma

    Plasmid

    #8895

    Purpose

    Mammalian expression of flag-tagged PPAR gamma

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.2 TDP-43 NLS1, 5F-L YFP

    Plasmid

    #84913

    Purpose

    Mammalian expresion of TDP-43 NLS1, 5F-L YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Hygro(+)-PSTCD-cGAS

    Plasmid

    #102605

    Purpose

    Mammalian expression of cGAS fused to PSTCD tag

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge