We narrowed to 6,927 results for: 11

1. pGSTag BimL (T56A)

   Plasmid

   #24253

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   BimL(T56A) (BCL2L11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed threonine 56 to alanine

   Promoter

   Available since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pGSTag BimL (S58A)

   Plasmid

   #24254

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   BimL(S58A) (BCL2L11 Human)
   

   Use

   Tags

   Gst

   Expression

   Bacterial

   Mutation

   Changed serine 58 to alanine

   Promoter

   Available since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3 T7 BimL (S44A)

   Plasmid

   #24245

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   BimL (S44A) (BCL2L11 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Changed Serine 44 to Alanine

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

4. BSD-CryC

   Plasmid

   #209672

   Purpose

   Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cells

   Depositor

   Sanjeevi Sivasankar

   Article

   Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Lentiviral

   Tags

   Split-TurboID-C and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Pb-TRE3G-SpCas9-IRES-blast

   Plasmid

   #195506

   Purpose

   Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti6-SLC7A11/xCT-V5

   Plasmid

   #170427

   Purpose

   Eepresses SLC7A11 (also named xCT) in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

   Insert

   solute carrier family 7 member 11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21a - TR

   Plasmid

   #89621

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Thioredoxin
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-Cas9-2A-GFP-sgRNA

   Plasmid

   #124889

   Purpose

   Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

   Depositor

   Harvey Lodish

   Article

   Li et al Haematologica. 2016 Jun;101(6):e216-9. d

   Insert

   Cas9
   

   Use

   Retroviral

   Tags

   2A-GFP

   Expression

   Mutation

   Promoter

   MSCV

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 3xFLAG-dCas9/pCMV-7.1

   Plasmid

   #47948

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A (catalyticall…

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

    Plasmid

    #89608

    Purpose

    Expression of Luciferase in human cells. 3rd generation lentiviral vector

    Depositor

    Fernando Fierro

    Article

    Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MNDU3

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPR-V2_hMSH2

    Plasmid

    #186155

    Purpose

    sgRNA targeting human MSH2 gene

    Depositor

    David McFadden

    Article

    Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

    Insert

    MSH2 (MSH2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid

    #129631

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-TRE3G-PYL1-sfGFP-PML

    Plasmid

    #167011

    Purpose

    expression of PYL1-SfGFP-PML, lentivirus vector

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    PML (PML Human)
    

    Use

    Lentiviral

    Tags

    GFP-PML

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

    Plasmid

    #166055

    Purpose

    Expresses the full length TurboID on the surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcVpx.myc

    Plasmid

    #213388

    Purpose

    Generate lentiviral vector which increases gene delivery into primary human myeloid cells

    Depositor

    Nathaniel Landau

    Article

    Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

    Insert

    SIVmac vpx (vpx Simian Immunodeficiency Virus)
    

    Use

    Lentiviral

    Tags

    myc-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-p38alpha

    Plasmid

    #86832

    Purpose

    mammalian expression of GFP-p38alpha

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38alpha (MAPK14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-4T-1 3xFlag-JNK1a1

    Plasmid

    #47574

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    JNK1a1 (MAPK8 Human)
    

    Use

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

19. AAV-hSynI-V5-TurboID(N)-GPI

    Plasmid

    #163696

    Purpose

    Expresses n-terminal half of TurboID on surface of neurons

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

    Plasmid

    #163697

    Purpose

    Expresses c-terminal half of TurboID on surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC19-Hph

    Plasmid

    #188741

    Purpose

    pUC19 plasmid (MCS intact) with Hygromycin selection marker

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    Hygromycin
    

    Use

    Filamentous fungal expression

    Tags

    Expression

    Mutation

    Promoter

    gpdA

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Flag-FOXM1

    Plasmid

    #153136

    Purpose

    Expression of Flag tagged human FOXM1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXM1 (FOXM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HSC1-GiP

    Plasmid

    #58254

    Purpose

    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

    Plasmid

    #129632

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLNHA-C1-HsXRN1

    Plasmid

    #66596

    Purpose

    expresses lambdaN-HA-tagged XRN1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    XRN1 (XRN1 Human)
    

    Use

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET30a-AlkB-D135S

    Plasmid

    #79051

    Purpose

    Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSFDuet-HIS-FLAG-AtUBQ11

    Plasmid

    #222720

    Purpose

    Expression HIS-FLAG-AtUBQ11 in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Insert

    AtUBQ11 (UBQ11 Mustard Weed)
    

    Use

    Tags

    2xFLAG and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET30a-AlkB

    Plasmid

    #79050

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lyn11-CIBN-GFP

    Plasmid

    #79572

    Purpose

    expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    Lyn11
    

    Use

    Tags

    CIBN (CRY2-binding domain) and EGFP

    Expression

    Mammalian

    Mutation

    G8R (please see depositor comments below)

    Promoter

    CMV

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

30. Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

    Plasmid

    #163029

    Purpose

    FLiCRE TF for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    Nrxn3b-MKII-hLOV1-TEVcs-tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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