Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,964 results for: BASE

Clear keywords
Showing: 7881 - 7920 of 12964 results

  1. pcDNA3.1 Flag YFP hNKCC1 C295S (NT103)

    Plasmid
    #49066
    Purpose
    Expresses human NKCC1 C295S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295S in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Nov. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    49066-plasmid-map-sequence-id-72192

    Enlarge

  17. pHyperTEV60

    Plasmid
    #228496
    Purpose
    10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    TEV Protease
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228496-plasmid-map-sequence-id-455395

    Enlarge

  18. pUC-L3L2

    Plasmid
    #114019
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114019-plasmid-map-sequence-id-222772

    Enlarge

  19. pUC-L1L4

    Plasmid
    #114018
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114018-plasmid-map-sequence-id-222765

    Enlarge

  20. lis-1::L4440

    Plasmid
    #11336
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    RNAi
    Expression
    Worm
    Available Since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  21. nud-1::L4440

    Plasmid
    #11337
    Depositor
    Guy Caldwell
    Insert
    nud-1 (nud-1 Nematode)
    Use
    RNAi
    Expression
    Worm
    Available Since
    July 12, 2006
    Availability
    Academic Institutions and Nonprofits only

  22. pUC-L1L2

    Plasmid
    #114017
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114017-plasmid-map-sequence-id-222768

    Enlarge

  23. pMH_MBP_AtSOG1_54-499

    Plasmid
    #238518
    Purpose
    Bacterial expression of A. thaliana SOG1 54-499
    Depositor
    Michael Hothorn
    Insert
    AtSOG1
    Tags
    2x Strep, 6x His, MBP, N-term, TEV cleavable
    Expression
    Bacterial
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238518-plasmid-map-sequence-id-477132

    Enlarge

  24. MZB186

    Plasmid
    #228489
    Purpose
    Empty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    10xHis-SUMO(Smt3)-Avitag
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228489-plasmid-map-sequence-id-455379

    Enlarge

  25. pJG01

    Plasmid
    #213505
    Purpose
    expresses TdTomato in Capsaspora owczarzaki
    Depositor
    Joseph Gerdt
    Insert
    TdTomato
    Promoter
    EF1
    Available Since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    213505-plasmid-map-sequence-id-431905

    Enlarge

  26. PB513B-1/TRE-hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ-EF1α-Puro

    Plasmid
    #199550
    Purpose
    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoter
    Depositor
    Masamichi Kamihira
    Article
    Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33
    Insert
    hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ (HNF1A, FOXA2, CEBPB, CEBPA, CEBPG Human)
    Use
    Piggybac transposon vector
    Expression
    Mammalian
    Promoter
    TRE+CMVmin promoter
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    199550-plasmid-map-sequence-id-405449

    Enlarge

  28. pLS-SV40-mP-Rluc

    Plasmid
    #106292
    Purpose
    Luciferase lentivirus based enhancer assay vector
    Depositor
    Nadav Ahituv
    Insert
    Luciferase
    Use
    Lentiviral and Luciferase
    Promoter
    Derived from pGL4.23
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    106292-plasmid-map-sequence-id-212597

    Enlarge

  29. pOPINN-GFP

    Plasmid
    #53541
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Tags
    N-His-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53541-plasmid-map-sequence-id-83419

    Enlarge

  30. pFA6a-SRM (sortase recognition motif)-hphMX4

    Plasmid
    #54488
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Eri Sakata
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    hphMX4
    Available Since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. MZB211

    Plasmid
    #228494
    Purpose
    10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    pol
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    A408S
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228494-plasmid-map-sequence-id-455979

    Enlarge

  32. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

    Enlarge

  33. pBIR-GFP

    Plasmid
    #49807
    Purpose
    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.
    Depositor
    Thanos Halazonetis
    Insert
    GFP
    Expression
    Mammalian
    Promoter
    beta-actin
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49807-plasmid-map-sequence-id-74303

    Enlarge

  34. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. MZB174

    Plasmid
    #228487
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    10xHis-SUMO(Smt3)-GST-HRV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228487-plasmid-map-sequence-id-455394

    Enlarge

  36. Col2a1:TFPnls-mErt-Cre-Ert

    Plasmid
    #111152
    Purpose
    Chondrocyte specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-mErt-Cre-Ert
    Use
    Cre/Lox
    Mutation
    wt
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    111152-plasmid-map-sequence-id-211539

    Enlarge

  37. pC23-LIC-STREP

    Plasmid
    #182507
    Purpose
    pET based vector for protein expression in E.coli for targets fused with C-terminal STREP
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Tags
    TEV-STREP
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182507-plasmid-map-sequence-id-361392

    Enlarge

  38. pC28-LIC-His

    Plasmid
    #182504
    Purpose
    pET based vector for protein expression in E.coli for targets fused with C-terminal His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Tags
    TEV-6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182504-plasmid-map-sequence-id-358072

    Enlarge

  39. pMH_AtCK2alpha1_76-409_HC

    Plasmid
    #238511
    Purpose
    Bacterial expression of A. thaliana CK2alpha1 76-409_HC
    Depositor
    Michael Hothorn
    Insert
    AtCK2alpha1 (CKA1 Mustard Weed)
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238511-plasmid-map-sequence-id-477127

    Enlarge

  40. pCfb13198

    Plasmid
    #219873
    Purpose
    The base plasmid of TUNEYALI forTF08
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08
    Expression
    Yeast
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    219873-plasmid-map-sequence-id-467444

    Enlarge

Showing: 7881 - 7920 of 12964 results