We narrowed to 24,606 results for: Nes

1. pHygromycin (GB0211)

   Plasmid

   #68204

   Purpose

   Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Hygromycin resistance gene coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGE-attBTT-loxP-DsRed (pTL780)

   Plasmid

   #115160

   Purpose

   Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

   Depositor

   Tobias Langenhan

   Article

   Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

   Insert

   attB-TT loxP 3xP3-DsRed
   

   Use

   Cre/Lox

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hCMV-IE1:GLuc:bgH

   Plasmid

   #135294

   Purpose

   Transcriptional unit for constitutive expression of GLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:GLuc:bGH
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLuciferase (GB0096)

   Plasmid

   #68201

   Purpose

   Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Luciferase coding region
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Orange Nano-lantern/pcDNA3

   Plasmid

   #67661

   Purpose

   orange luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Orange Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. c3GIC9n

   Plasmid

   #62192

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9 (D10A)
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mutation

   D10A substitution (Cas9n)

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cyan Nano-lantern/pcDNA3

   Plasmid

   #67660

   Purpose

   cyan luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Cyan Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLIK 3xFLAG-TWIST2 neo

   Plasmid

   #197936

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCerulean3-H2B-6

   Plasmid

   #55421

   Purpose

   Localization: Nucleus/Histones, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pJG85

    Plasmid

    #89281

    Purpose

    Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TB:7xSMAD_RE:MiniP

    Plasmid

    #133879

    Purpose

    7 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies the SMAD DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 shTGFBR3 puro

    Plasmid

    #58696

    Purpose

    Lentiviral shRNA vector for knockdown of human TGFBR3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    shTGFBR3 (TGFBR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

13. p5xE-Box

    Plasmid

    #118052

    Purpose

    GBPart - Operator/DNA Response element - 5 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    5 copies of the E-box motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKMD106h - intein-Cas9(C574)

    Plasmid

    #64191

    Purpose

    Expresses intein-Cas9(C574) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221-SMAD3

    Plasmid

    #79507

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

    Plasmid

    #87350

    Purpose

    S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/S355C/C574S/S867C

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TET-pLKO.1 PURO shSMAD4 #2

    Plasmid

    #83091

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human SMAD4

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shSMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pSLIK_hygro_3xflag_Luciferase

    Plasmid

    #136528

    Purpose

    Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    Luciferase
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB3035(X-4 MarkerFree)

    Plasmid

    #73273

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 35S::Cel1SP-GeNL/pCAMBIA1301

    Plasmid

    #190069

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Cel1 signal peptide-Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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