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Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
p6621 MSCV-IP N-HAonly SRC

Plasmid

#35017

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

SRC (SRC Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pNXgate32-3HA

Plasmid

#105076

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET32a-HD39Q

Plasmid

#11514

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 39 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 39 glutamines, vector m…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delZn3

Plasmid

#173936

Purpose

Bacterial expression of PARP1 lacking Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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hGATA-1 K137R

Plasmid

#118353

Purpose

This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K137R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199195

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199196

Purpose

Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TRAIL-R4

Plasmid

#61385

Purpose

Expresses HA tagged TRAIL receptor 4

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 4 (TNFRSF10D Human)

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R4 leader peptide (aa1-29) replaced with TN…

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pBS-AC3CtermGenomic-mCherry

Plasmid

#105250

Purpose

mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRSpuro shID3#1

Plasmid

#19163

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
mcherry Gamma 9 ---pcDNA3.1

Plasmid

#64203

Purpose

G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

G protein Gamma 9 (GNGT2 Human)

Tags

6xHis and mcherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pBabe HA human Bmf

Plasmid

#17239

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (BMF Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pET48 AtGUN1-SMR1

Plasmid

#136361

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR2

Plasmid

#136362

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR1

Plasmid

#136359

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR2

Plasmid

#136360

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-EIF2AK2-ts1

Plasmid

#174247

Purpose

EIF2AK2 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

EIF2AK2 shRNA (EIF2AK2 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLINK cyclin T1 (1-280) myc (K93D) (P#580)

Plasmid

#14611

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) K93D (CCNT1 Human)

Tags

myc

Expression

Mammalian

Mutation

K93D. Deficient in binding to CDK9. Inactive in 3…

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pTET-IUPi

Plasmid

#122019

Purpose

Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Inserts

Choline kinase (CKI1 Budding Yeast)
Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
isopentenyl-diphosphate Delta-isomerase

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pTET

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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