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pcDNA3-Akt-STOPS-NLS

Plasmid

#175007

Purpose

Nuclear-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_AC

Plasmid

#148417

Purpose

Mammalian Expression of HsRck

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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cjBlue chromoprotein

Plasmid

#117844

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, cjBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-STOPS-NES

Plasmid

#175008

Purpose

Cytosol-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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meffRed chromoprotein

Plasmid

#117836

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, meffRed

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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p5070 pcDNA4c hBrd4 (1047-1362)

Plasmid

#14449

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1047-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD)

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
p5069 pcDNA4c hBrd4 (471-730)

Plasmid

#14448

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (471-730) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 471-730

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
p5079 pcDNA4c hBrd4 (731-1046)

Plasmid

#14455

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (731-1046) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 731-1046

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
RHBDD1 pmRFP-N2 (1390)

Plasmid

#62049

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
p5071 pcDNA4c hBrd4 (595-1362)

Plasmid

#14450

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (595-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 595-1362

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein promoter-less

Plasmid

#118006

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD_Ub11TCA

Plasmid

#174517

Purpose

Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

Ubiquitin

Tags

6xHis

Expression

Bacterial

Mutation

Recoded with TCA replacing codon 11 of wt ubiquit…

Promoter

araBAD

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHM123DEnv

Plasmid

#137668

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein promoter-less

Plasmid

#118007

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EBP1_Z

Plasmid

#148150

Purpose

Mammalian Expression of Hs4EBP1

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

Hs4EBP1 (EIF4EBP1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNXF-1-5xmut_O

Plasmid

#147116

Purpose

Mammalian Expression of HsNxf-1-5xmut

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HsNxf-1-5xmut (NXF1 Human)

Expression

Mammalian

Mutation

See Depositor Comments Below

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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p6346 MSCV-CMV-Flag-HA-Brd4-1-444

Plasmid

#32886

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 (BRD4 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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p5072 pcDNA4c hBrd4 (1224-1362)

Plasmid

#14451

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1224-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1224-1362

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
p5082 pcDNA4c hBrd4 (1047-1133)

Plasmid

#14458

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1047-1133) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1047-1133

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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p5080 pcDNA4c hBrd4 (1134-1362)

Plasmid

#14456

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1134-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1134-1362

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only