We narrowed to 170,492 results for: addgene

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p8270 LentiCRISPR v2 hygro sgSIGIRR-3

Plasmid

#193979

Purpose

Expression of spCas9 and sgRNA targeting SIGIRR

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)

Use

Lentiviral

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-tevopreq1-FXR1-G266E

Plasmid

#225482

Purpose

Express epegRNA for FXR1

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

epegRNA for editing FXR1-G266E (FXR1 Human)

Use

CRISPR

Promoter

hU6

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LsgRNA-FXR1-KH1-gRNA

Plasmid

#225483

Purpose

Express gRNA for FXR1

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

gRNA for editing FXR1-KH1 (FXR1 Human)

Use

CRISPR

Promoter

hU6

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520-FXR1-N202S-gRNA

Plasmid

#225481

Purpose

Express gRNA for FXR1

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

gRNA for editing FXR1-N202S (FXR1 Human)

Use

CRISPR

Promoter

hU6

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLVUT-eC3GFP

Plasmid

#225485

Purpose

Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Exoenzyme C3 transferase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 30-251, See Depositor Comments

Promoter

Human ubiquitin C

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pST39-Mega'

Plasmid

#216877

Purpose

Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Derived from Mega, and GvpS and GvpK overlap has been resolved

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p8391 LentiCRISPRv2 Neo sgPTPN14-1

Plasmid

#221651

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-1 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8392 LentiCRISPRv2 Neo sgPTPN14-3

Plasmid

#221652

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-3 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNLS-VP16-NIR-Fb(718)

Plasmid

#220745

Purpose

NLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-Fb(718)

Use

Affinity Reagent/ Antibody

Tags

NLS-VP16

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG16

Plasmid

#220749

Purpose

NIR-FbLAG16 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG16

Use

AAV and Affinity Reagent/ Antibody

Promoter

CAG

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG30

Plasmid

#220750

Purpose

NIR-FbLAG30 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

AAV and Affinity Reagent/ Antibody

Promoter

CAG

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-CDK8

Plasmid

#222609

Purpose

Expression of MAC3-tagged CDK8

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

CDK8 (CDK8 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Tags

SNAP

Expression

Bacterial and Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL390

Plasmid

#219500

Purpose

Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT

Tags

SV40-NLS

Expression

Yeast

Promoter

GAL1-GAL10

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL391

Plasmid

#219501

Purpose

Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 2

Plasmid

#207610

Purpose

sgRNA 2 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

TCAGGCCGCAGGAAGAGGAA

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 1

Plasmid

#207609

Purpose

sgRNA 1 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

ACCCTAACTGAGAAGGGCGT

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-SHmOCR

Plasmid

#206874

Purpose

Empty Gateway pDEST vector for bacterial expression with Super His - Monomeric OCR fusion

Depositor

Murray Junop

Article

Szabla et al Nucleic Acids Res. 2024 Jul 22:gkae635.

Type

Empty backbone

Tags

HSV + 6xHis and SuperHis (14xHis) + monomeric OCR…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(143-421)msGFP

Plasmid

#209015

Purpose

Overexpression of truncated syt1 tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 143-421 only

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-C-TelR

Plasmid

#204981

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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