We narrowed to 14,583 results for: pET

1. pET-22b(+)_PelB-BN00H3-S-FLAG-His

   Plasmid

   #218038

   Purpose

   RESOLUTE SLC binder expression plasmid

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Insert

   BN00H3-S
   

   Use

   Tags

   1xFLAG 10xHis and PelB

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-22b(+)_PelB-BN00FH-2-FLAG-His

   Plasmid

   #218037

   Purpose

   RESOLUTE SLC binder expression plasmid

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Insert

   BN00FH-2
   

   Use

   Tags

   1xFLAG 10xHis and PelB

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-22b(+)_PelB-BN00B0-E-FLAG-His

   Plasmid

   #218035

   Purpose

   RESOLUTE SLC binder expression plasmid

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Insert

   BN00B0-E
   

   Use

   Tags

   1xFLAG 10xHis and PelB

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

   Plasmid

   #218198

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM

   Depositor

   Florence Pojer

   Insert

   EndoCOM
   

   Use

   Tags

   3C-CBD-His

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 19-315

   Promoter

   T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-29b(+)_notufA-EmEndoF2-CBD-His

   Plasmid

   #218197

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2

   Depositor

   Florence Pojer

   Insert

   EndoF2
   

   Use

   Tags

   CBD-His

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 45-335

   Promoter

   T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-Keyhole limpet hemocyanin [N132A/12R]

   Plasmid

   #206532

   Purpose

   Mammalian Expression Plasmid of anti-Keyhole limpet hemocyanin (Giant keyhole limpet). Derived from hybridoma N132A/12.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Keyhole limpet hemocyanin (Megathura crenulata) recombinant Mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET SUMO-syt7 (136-403) L361C

   Plasmid

   #213622

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 (136-403) L361C

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 (136-403) L361C (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   C260A C275A L361C

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET 15b+His SUMO Rab12 (Q101L)

   Plasmid

   #208371

   Purpose

   Bacterial expression of His-SUMO-tagged human Rab12 (Q101L)

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   His SUMO Rab12 (Q101L) (RAB12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q101L

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-Duet-6xHis-TEV-Nsp8(76-198)

   Plasmid

   #201024

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDUET-GST-AUP1 379-410:Ube2G2

    Plasmid

    #185335

    Purpose

    Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM-60-NcQDE-2_506-938-del787-838GSG_K

    Plasmid

    #146730

    Purpose

    Bacterial Expression of NcQde-2_506-938-del787-838GSG

    Depositor

    Elisa Izaurralde

    Insert

    NcQde-2_506-938-del787-838GSG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-6xhis-sumo-CeMIGA(106-496)

    Plasmid

    #192832

    Purpose

    Express C.elegans MIGA fragment 106-496 in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Mitoguardin homologue (miga-1 Nematode)
    

    Use

    Tags

    6xhis sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. human KANK2-A670V Ankyrin Repeats pET151

    Plasmid

    #164055

    Purpose

    Expresses human KANK2 Ank domain - A670V mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mouse KANK4-Y801H Ankyrin Repeats pET151

    Plasmid

    #164058

    Purpose

    Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet1-LanCL2-C321A/C367A-C-Ter-His

    Plasmid

    #154188

    Purpose

    To express LanCL2-C321A/C367A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Cys321->Ala321; Cys367->Ala367

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

    Plasmid

    #169499

    Purpose

    Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRBSA

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

    Plasmid

    #169498

    Purpose

    Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-His10-BTLAicd (AA190-289)-TwinStrep

    Plasmid

    #180805

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289)-TwinStrep (BTLA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CEA-N domain residues 1-132 (CEACAM5)-pET30b

    Plasmid

    #174693

    Purpose

    Plasmid expressing CEA-N domain

    Depositor

    Jean Gariepy

    Article

    Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

    Insert

    CEA-N Domain
    

    Use

    Tags

    TEV Cleavage Site and hexahistidine (HIS) tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 Promoter

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid

    #176573

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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