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Showing: 5941 - 5964 of 5964 results

  1. pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

    Plasmid
    #131684
    Purpose
    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1
    Depositor
    Christopher Richie
    Article
    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10
    Inserts
    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    Use
    AAV
    Tags
    KASH
    Expression
    Mutation
    Promoter
    EF1a and mU6-LSL (Cre dependent)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    131684-plasmid-map-sequence-id-437146

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  2. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234370
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234370-plasmid-map-sequence-id-469430

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  3. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234372
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234372-plasmid-map-sequence-id-469423

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  4. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

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  22. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

    Plasmid
    #209787
    Purpose
    Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)
    Depositor
    Yuxuan Guo
    Article
    Yang et al Circulation. 2024 May 28;149(22):1778-17
    Insert
    TNT4, TadA8e, SpRY N, inteinN, miR122 TS
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    TNT4
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209787-plasmid-map-sequence-id-416840

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  23. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid
    #207355
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207355-plasmid-map-sequence-id-408416

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  24. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Expression
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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Showing: 5941 - 5964 of 5964 results