We narrowed to 23,128 results for: Sis

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pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

Plasmid

#173462

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

Plasmid

#173460

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-mNeonGreen (pJJF003)

Plasmid

#173459

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

Plasmid

#173458

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pThuAsol

Plasmid

#171675

Purpose

pThuAsol expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

SUMO-TEV-ThuAsol

Tags

His-tag and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO chicken-cPLA2-C2-domain

Plasmid

#170753

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

Insert

PLA2G4A-C2 domain (PLA2G4A Chicken)

Tags

SUMO

Expression

Bacterial

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-Gal80

Plasmid

#169030

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59Q, TadA* V106W)

Plasmid

#126496

Purpose

Expresses ABEmax(TadA E59Q, TadA*V106W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59Q TadA* V106W)

Expression

Mammalian

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59, TadA* V106W)

Plasmid

#126497

Purpose

Expresses ABEmax(TadA E59, TadA*V106W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59 TadA* V106W)

Expression

Mammalian

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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AP-VAMP7(low)

Plasmid

#167998

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP7(K121-R180)-T7PAP

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP1

Plasmid

#168022

Purpose

Expresses eCFP-VAMP1(T29-K89)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP1(T29-K89)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP4

Plasmid

#168025

Purpose

Expresses eCFP-VAMP4(P48-R107)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP4(P48-R107)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP3

Plasmid

#168024

Purpose

Expresses eCFP-VAMP3(S11-R69)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP3(S11-R69)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP7

Plasmid

#168027

Purpose

Expresses eCFP-VAMP7(K121-R180)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP7(K121-R180)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP5

Plasmid

#168026

Purpose

Expresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP5(M1-Q60)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWL89A mmPing20F

Plasmid

#145789

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Expression

Yeast

Mutation

Insertion of the FMV enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20B

Plasmid

#145790

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20B

Expression

Yeast

Mutation

Insertion of the b-Conglycin Enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A NL60

Plasmid

#145791

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

NL60

Expression

Yeast

Mutation

Insertion of the OCS Enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWL89A 2xE

Plasmid

#145792

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

2xE

Expression

Yeast

Mutation

Insertion of 2 copies of the CMV Enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWL89A 4xE

Plasmid

#145793

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

4XE

Expression

Yeast

Mutation

Insertion of 4 copies of the CMV Enhancer

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-tetpA(R1R4)-iRPT

Plasmid

#154882

Purpose

Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2

Expression

Mammalian

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw

Plasmid

#162479

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw

Plasmid

#162474

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

CreBC

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HJP-473-DNE>FLP-T2A-CreAB-NrdJ-1N

Plasmid

#162480

Purpose

This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

DNE-Frterminator

Use

Cre/Lox

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-C2X -MTalpha-FliG

Plasmid

#154361

Purpose

Bacterial expression of Mtalpha

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Mtalpha

Expression

Bacterial

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-C2X -MTn-FliG

Plasmid

#154360

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Expression

Bacterial

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
as-mOPN-PB0

Plasmid

#106450

Purpose

Bacterial expression of antisense to murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

antisense-osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

antisense

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-C2X dAmp/Kana+-AFP

Plasmid

#154359

Purpose

Bacterial expression of AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

AFP

Tags

His

Expression

Bacterial

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE477

Plasmid

#153268

Purpose

M3 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE478

Plasmid

#153269

Purpose

M4 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE501

Plasmid

#153274

Purpose

M7 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE499

Plasmid

#153272

Purpose

M6 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE498

Plasmid

#153271

Purpose

M5 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-C2X -2AFP-FliG

Plasmid

#154362

Purpose

Bacterial expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Expression

Bacterial

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-C2X dAmp/Kana+-2AFP

Plasmid

#154358

Purpose

Bacterial expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Tags

His

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet1-tglCDEF

Plasmid

#134677

Purpose

Expresses TglC, TglD, TglE, TglF in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Inserts

TglC
TglD
TglE
TglF

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet1-ammA(∆Trp56)

Plasmid

#134683

Purpose

Expresses His6-AmmA(∆Trp56) in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA(delW56)

Tags

His6

Expression

Bacterial

Mutation

deletion of W56

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-GFP-tglG

Plasmid

#134679

Purpose

Expresses His6-GFP-TglG fusion in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglG

Tags

His6-GFP

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-tglHI

Plasmid

#134672

Purpose

Expresses His6-TglH and TglI in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Inserts

TglH
TglI

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR207-OEP7(1–50)

Plasmid

#100601

Purpose

Gateway entry vector for AtOEP7(1–50)

Depositor

Yutaka Kodama

Article

Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

Insert

OEP7(1–50) (OEP7 Mustard Weed)

Use

Gateway entry vector

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN-D2irTNG5kwh

Plasmid

#128279

Purpose

Mammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion protein

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

hTetR::NLS::EGFP

Use

Synthetic Biology; Flp-in expression vector

Tags

EGFP

Expression

Mammalian

Promoter

CMV-D2ir promoter

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_024

Plasmid

#123836

Purpose

Encodes Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

ABI

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-1/3/2

Plasmid

#131026

Purpose

Three-spacer array 2 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-1/2/3

Plasmid

#131027

Purpose

Three-spacer array 1 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-2

Plasmid

#131025

Purpose

Single spacer array with spacer #2 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-3

Plasmid

#131024

Purpose

Single spacer array with spacer #3 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFncpf1_7_spacer_array

Plasmid

#131032

Purpose

Seven-spacer array for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

7-spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-3/2/1

Plasmid

#131031

Purpose

Three-spacer array 6 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-2/3/1

Plasmid

#131030

Purpose

Three-spacer array 5 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcF-3/1/2

Plasmid

#131029

Purpose

Three-spacer array 4 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

3-spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 23,128 results for: Sis