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pET28-p53 (1-73) (K24N)

Plasmid

#62079

Purpose

Expression of human p53 (1-73) (K24N) in e. coli

Depositor

Gary Daughdrill

Article

Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-73; K24N

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a S620T

Plasmid

#53059

Purpose

Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Serine 620 to Threonine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a S631A

Plasmid

#53058

Purpose

Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Serine 631 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pX41

Plasmid

#46851

Purpose

TOP Hupki-wt plasmid without selection marker

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB and Hupki p53 wild type (TP53 Mouse, Human)

Expression

Mammalian

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2c-E471Q

Plasmid

#45704

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E471Q

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only
pM-ErbB4-delta-kinase-PY1 mutant

Plasmid

#17801

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP/CALR del52

Plasmid

#214700

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAX APP Swe/Ind

Plasmid

#30145

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

human APP 695 Swedish/Indiana mutation (APP Human)

Expression

Mammalian

Mutation

Swedish and Indiana; K595N, M596L and V642F

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-N-Drd1

Plasmid

#104358

Purpose

Expresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.

Depositor

Kirk Mykytyn

Article

Domire et al Cell Mol Life Sci. 2011 Sep;68(17):2951-

Insert

Dopamine receptor D1 (Drd1) (Drd1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5B*-strep

Plasmid

#120164

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE4

Plasmid

#197148

Purpose

Expresses ApoE4 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE4 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pcDNA3

Plasmid

#18955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pVax1-hVEGF165

Plasmid

#74466

Purpose

expression of hVEGF165 in mammalian cells

Depositor

Loree Heller

Article

Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

Insert

vascular endothelial growth factor 165 (VEGFA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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ADRA1A-Tango

Plasmid

#66213

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADRA1A (ADRA1A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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mouse b8 full length

Plasmid

#217817

Purpose

Full length mouse integrin b8 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGB8 (Itgb8 Mouse)

Tags

P2A-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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mouse a5 full length

Plasmid

#217810

Purpose

Full length mouse integrin a5 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGA5 (Itga5 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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MGAT2-RpHLuorin2

Plasmid

#171718

Purpose

Cis-/medial-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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ptf-Galectin3

Plasmid

#64149

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Tags

EGFP and mRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-Gal3

Plasmid

#85662

Purpose

Fluorescent reporter for lysosomal damage

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

LGALS3 (LGALS3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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