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pET21d-colEwt2/Imwt2

Plasmid

#107127

Purpose

plasmid for expression of WT colicin endonuclease and immunity pair 2

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEwt2/Imwt2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDHC29

Plasmid

#80781

Purpose

Cloning vector compatible with ColE1 plasmids with high copy number

Depositor

Gregory Phillips

Article

Phillips et al Biotechniques. 2000 Mar;28(3):400-2, 404

Type

Empty backbone

Expression

Bacterial

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G04

Plasmid

#67138

Purpose

Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

EGFP and HIS

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-d2eGFP-16pf

Plasmid

#21969

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

CMV d2eGFP miR-16 perfect

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only
pLenti6.3-SlugCas9-BlastR

Plasmid

#220965

Purpose

Expresses human codon-optimized SlugCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SlugCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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ART_RTctrl

Plasmid

#214066

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCellFree_G07

Plasmid

#67141

Purpose

Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pRB35

Plasmid

#72866

Purpose

template base vector for N-terminal tagging, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for N-terminal tags, Puro

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(RK)

Plasmid

#27136

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Arginines in the SH2 domains mutated to lysines (…

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-S

Plasmid

#154415

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of s…

Mutation

Many synonymous changes due to codon optimization

Available Since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

Plasmid

#154029

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Mutation

Many synonymous changes due to codon optimization

Available Since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-HLA-B0702-His

Plasmid

#104849

Purpose

HLA-B0702 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-B Human)

Use

Entry vector for gateway system

Tags

his

Promoter

none

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)

Plasmid

#29446

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 520-1556 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
FpG5.BFP.Reporter

Plasmid

#134967

Purpose

Lentiviral vector for enhancer reporter assay

Depositor

Beat Bornhauser

Article

Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

mini Fgf4 promoter

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG024

Plasmid

#113991

Purpose

Level 1 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-Renilla

Plasmid

#79496

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Renilla Luciferase

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGGA006

Plasmid

#48816

Purpose

Provides the UBQ10 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pT-60

Plasmid

#16090

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biochem (Tokyo). 2003 Apr . 133(4):415

Insert

UBC9 (UBC9 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pYC01

Plasmid

#224586

Purpose

IPTG-inducible phage φX174 lysis gene E

Depositor

Ophelia Venturelli

Article

Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

Insert

phage φX174 lysis gene E (phiX174p08 )

Use

Synthetic Biology

Expression

Bacterial

Promoter

PA1lacO-1

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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