We narrowed to 8,634 results for: dykddddk
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Plasmid#140012PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianMutationY126APromoterEF1Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y419F
Plasmid#140317PurposeExpresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studiesDepositorAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 Flag-Apex-Rab7A
Plasmid#135652PurposeRab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab7A (RAB7A Human)
TagsApex2 and FLagExpressionMammalianPromoterL30Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsCERK
Plasmid#118600PurposeExpresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphateDepositorInsertHomo sapiens ceramide kinase
TagsFLAGExpressionYeastPromoterGAL1, 10Available sinceFeb. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry
Plasmid#135014PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the C-termi…PromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-(BsaI)_CBh-Cas9-T2A-mCherry
Plasmid#135012PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianPromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG
Plasmid#132682PurposeAAV plasmid encoding mouse PM20D1 with C-terminal flag tagDepositorInsertPeptidase M20 domain containing 1 (Pm20d1 Mouse)
UseAAVTags6xHIS and FlagPromoterChicken b-actinAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
Plasmid#133779PurposeExpresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (IDH1 Human)
TagsMyc and FLAGExpressionMammalianMutationArginine is mutated to histidine at amino acid po…Available sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor TagBFP2-3XFlag (cyto) WPRE
Plasmid#129421PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter proteinDepositorInsertTagBFP2-3Flag
UseMosaic analysis for dual recombinase-mediated cas…Tags3X Flag epitope tagPromoternone (3X polyA to mitigate episomal expression)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA6-N-3XFLAG-Gp78
Plasmid#123590DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertautocrine motility factor receptor (Amfr Mouse)
TagsFLAGExpressionMammalianAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ΔN-RNMT
Plasmid#112708PurposeExpresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNMT (RNMT Human)
TagsFLAG and HIV Rev nuclear export signal (NES)ExpressionMammalianMutationdeleted amino acids 2-120, mutated active-site D2…PromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA6-N-3XFLAG-Ubac2
Plasmid#123597DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertubiquitin associated domain containing 2 (Ubac2 Mouse)
TagsFLAGExpressionMammalianAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB-bsd-Flag-Trl1
Plasmid#126609PurposeExpresses N-terminal Flag-tagged yeast Trl1DepositorAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-G185R
Plasmid#125849Purposeexpress mutant BAP1-G185RDepositorAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-LLAA(20)
Plasmid#124397PurposeFull-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
TagsFlagExpressionMammalianMutationL897A/L900A mutations (full length protein is 213…Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAM105
Plasmid#86479PurposeExpresses p9-FLAG-FMODepositorPublicationInsertp9-FLAG-FMO
UseSynthetic BiologyTagsFLAG and FMOExpressionBacterialPromoterpTetAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/SCAF4-FLAG
Plasmid#122469PurposeDox-inducible FLAG (C-terminal tag) SCAF4 expressionDepositorAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G
Plasmid#113953Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, I132S, S212N, and …PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_CoumRS
Plasmid#64882PurposeExpresses Coumarinyl- tRNA synthetaseDepositorPublicationInsertCoum_RS
TagsFlagPromoterCMVAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNIA-CEN-FLAG-LINXa4(RLR)-Bre1
Plasmid#116884PurposeFLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutationsDepositorInsertLINXa4(RLR)-Bre1
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11A, LINXa3 wi…PromoterADH1Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by