We narrowed to 733 results for: Tcta

1. pk335-CARGO-6mer-55kb-USF

   Plasmid

   #227458

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 55kb Upstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pk335-CARGO-6mer-7.5kb-USP

   Plasmid

   #227448

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 7.5kb Upstream Prdm8
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. p426_Cas9_gRNA-CAN1y

   Plasmid

   #87391

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting CAN1y sequence GATACGTTCTCTATGGAGGA in yeast chromosome 6.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting CAN1y
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCHKO_SFRS7_exon_deletion_3

   Plasmid

   #155071

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pk335-CARGO-11mer-35kb-DSF-1-11

   Plasmid

   #227489

   Purpose

   11-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 35kb Downstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiGuide-puro_Panc480-MT7

   Plasmid

   #200941

   Purpose

   Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   multiplexed sgRNA array
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF-1a

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pROS13-Tps2/Gsy1

   Plasmid

   #196613

   Purpose

   Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   S000001911 (TPS2, GSY1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCHKO_SFRS7_exon_deletion_2

   Plasmid

   #155070

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLCHKO_Ptbp_ex8_4

   Plasmid

   #155084

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. gh146

    Plasmid

    #106805

    Purpose

    expression of gRNA targeting FKTN

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FKTN (FKTN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCHKOv3-CD46 Ex3_3-Lb

    Plasmid

    #209029

    Purpose

    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & LbCas12a DR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCHKOv3-CD46 Ex3_3-As

    Plasmid

    #209033

    Purpose

    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting human CD46 Exon 3 for deletion using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    hgRNA for deletion of CD46 Exon 3 with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFlare9A-Clip2E9 Mutant

    Plasmid

    #209575

    Purpose

    minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Clip2 (Clip2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

    Promoter

    CMV

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLCHKO_TK1_HPRT1_Lb_2

    Plasmid

    #155060

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV CAG Barcode7

    Plasmid

    #229069

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    CAG Barcode7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV Syn Barcode15

    Plasmid

    #226189

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode15
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV Syn Barcode14

    Plasmid

    #226188

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode14
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV Syn Barcode13

    Plasmid

    #226187

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode13
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOpen-XbaIR

    Plasmid

    #165567

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    XbaI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAAV Syn Barcode20

    Plasmid

    #226192

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode20
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV Syn Barcode18

    Plasmid

    #226191

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode18
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV Syn Barcode17

    Plasmid

    #226190

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode17
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV Syn Barcode11

    Plasmid

    #226185

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode11
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTRERF1.1.0-gDNA

    Plasmid

    #176384

    Purpose

    Expresses spCas9 and gRNA sequence targeting TRERF1

    Depositor

    Barbara Stranger, Kevin White

    Insert

    TRERF1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLK88 - Edit-directing plasmid base

    Plasmid

    #98814

    Purpose

    Edit-directing plasmid base

    Depositor

    Leonid Kruglyak

    Insert

    CAN1.y gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pZBED9.1.2-gDNA

    Plasmid

    #175853

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZBED9-Nickase2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pROS15_MATz

    Plasmid

    #166100

    Purpose

    Expression of dual gRNA targeting the MAT locus

    Depositor

    Rebecca Burgess

    Insert

    MAT Z1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFG001

    Plasmid

    #62816

    Purpose

    Bacterial constitutive gRNA expression plasmid

    Depositor

    Sébastien Rodrigue

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PN25

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mU6-sgSik1_2nd-hU6-sgSik3_2nd

    Plasmid

    #177219

    Purpose

    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Insert

    sgSik1_2nd/sgSik3_2nd
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PLJR965_Rv1420

    Plasmid

    #241229

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1420) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1420 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available Since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LT3GEPIR-PSMB5shRNA

    Plasmid

    #220580

    Purpose

    To inducibly knockdown PSMB5 expression

    Depositor

    Sean Lee

    Insert

    PSMB5 shRNA (PSMB5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mU6-sgSik1_2nd-hU6-sgSik3_1st

    Plasmid

    #177218

    Purpose

    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Insert

    sgSik1_2nd/sgSik3_1st
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRHOXF2B.1.0-gDNA

    Plasmid

    #132462

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RHOXF2B (RHOXF2B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBsacA

    Plasmid

    #158650

    Purpose

    Constitutive transcription of sacA crRNA

    Depositor

    Wenjing Cui

    Insert

    sacA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pveg

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCfb13193

    Plasmid

    #219868

    Purpose

    The base plasmid of TUNEYALI for TF03

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF03 (YALI1_B05350g) and homologous arm matching TF03
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCfb13205

    Plasmid

    #219880

    Purpose

    The base plasmid of TUNEYALI for TF15

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF15 (YALI1_B29562g) and homologous arm matching TF15
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid

    #201402

    Purpose

    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    alpha-SMA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SGEP-sh-Hs-PHGDH-1968

    Plasmid

    #188673

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SGEP-sh-Hs-PHGDH-1967

    Plasmid

    #188672

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mU6-sgSik1_2nd-hU6-sgNT

    Plasmid

    #177213

    Purpose

    Expresses a Sik1-targeting (mU6) and a non-targeting (hU6) gRNAs and Cre recombinase

    Depositor

    Monte Winslow

    Insert

    sgSik1_2nd/sgNT1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge