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We narrowed to 446 results for: DHFR

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Showing: 51 - 100 of 446 results

  4. pDBD-M

    Plasmid
    #50572
    Purpose
    Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571
    Depositor
    Alexander Varshavsky
    Publication
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
    Expression
    Yeast
    Promoter
    Gal1
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Publication
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pLenti CMV Puro DHFR.dn-cHSF1

    Plasmid
    #134729
    Purpose
    Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain
    Depositor
    Matthew D. Shoulders
    Publication
    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.
    Insert
    DHFR.dn-cHSF1 (HSF1 Human)
    Use
    Lentiviral
    Tags
    DHFR
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 186-202, 379−529
    Promoter
    CMV
    Available since
    Aug. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    134729-plasmid-map-sequence-id-271703

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  32. nDHFR173-gs10-NLS-VPR

    Plasmid
    #188262
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nDHFR173 (DHFR Human)
    Expression
    Mammalian
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188262-plasmid-map-sequence-id-374570

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  34. dCas9-NLS-gs10-cDHFR174

    Plasmid
    #188256
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cDHFR174 (DHFR Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188256-plasmid-map-sequence-id-374594

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  41. TfR-moxGFP-DHFR

    Plasmid
    #173012
    Purpose
    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR-moxGFP-DHFR
    Tags
    DHFR and moxGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    173012-plasmid-map-sequence-id-339204

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  42. TfR-mCh-DHFR

    Plasmid
    #173013
    Purpose
    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR-mCh-DHFR
    Tags
    DHFR and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    173013-plasmid-map-sequence-id-339208

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  43. DHFR-mNeon-GluA1

    Plasmid
    #173009
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DHFR-mNeon-GluA1
    Tags
    DHFR and mNeon
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173009-plasmid-map-sequence-id-339113

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  45. DHFR-GFP-th-NL1

    Plasmid
    #173007
    Purpose
    For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DHFR-GFP-NL1
    Tags
    DHFR and EGFP
    Expression
    Mammalian
    Promoter
    human synapsin
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173007-plasmid-map-sequence-id-339111

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Showing: 51 - 100 of 446 results