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We narrowed to 705 results for: ELP

Showing: 51 - 100 of 705 results

  3. GFP-I48 ELP

    Plasmid
    #67857
    Purpose
    GFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residues
    Depositor
    Andrew MacKay
    Insert
    GFP fused to ELP (VPGIG) repeated 48 times
    Use
    Tags
    GFP fused to N-terminal of ELP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available Since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    67857-plasmid-map-sequence-id-117815

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  4. pNCMV 16E6no* delPDZ

    Plasmid
    #47678
    Purpose
    PDZ-binding motif is deleted
    Depositor
    Karl Munger
    Article
    Spangle et al J Virol. 2012 May 2.
    Insert
    HPV 16 E6no* (E6 HPV, Human)
    Use
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    del first 7aa and last 6aa, V42L*
    Promoter
    CMV
    Available Since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pNCMV 18E6no* delPDZ

    Plasmid
    #47887
    Purpose
    PDZ-binding motif is deleted
    Depositor
    Karl Munger
    Article
    Spangle et al J Virol. 2012 May 2.
    Insert
    HPV 18 E6no*
    Use
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    del last 6aa, V44A*
    Promoter
    CMV
    Available Since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  13. pNM220_AAVS1 integration helper

    Plasmid
    #211864
    Purpose
    Cas9 cutting at AAVS1 safe harbor locus
    Depositor
    Iain Cheeseman
    Insert
    AAVS1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    211864-plasmid-map-sequence-id-426338

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  26. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  27. pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

    Plasmid
    #168153
    Purpose
    Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB and TnsC)
    PmcTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168153-plasmid-map-sequence-id-330740

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  28. pMS1: pHelper(AvCAST)_AvPSP1

    Plasmid
    #168133
    Purpose
    Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168133-plasmid-map-sequence-id-330610

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  29. pMS18: pHelper(PmcCAST)_PmcPSP1

    Plasmid
    #168151
    Purpose
    Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    PmcTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168151-plasmid-map-sequence-id-330728

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  30. pMS23: pHelper(PmcCAST)_entry_ΔTnsD

    Plasmid
    #168156
    Purpose
    Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB and TnsC)
    PmcTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168156-plasmid-map-sequence-id-330720

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  31. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

    Plasmid
    #168135
    Purpose
    Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168135-plasmid-map-sequence-id-330693

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  33. pMS6: pHelper(AvCAST)_entry_ΔTnsD

    Plasmid
    #168139
    Purpose
    Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168139-plasmid-map-sequence-id-330862

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  46. pMS22: pHelper(PmcCAST)_entry_ΔTniQ

    Plasmid
    #168155
    Purpose
    Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168155-plasmid-map-sequence-id-330972

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  47. pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

    Plasmid
    #168152
    Purpose
    Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168152-plasmid-map-sequence-id-330917

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  48. pMS5: pHelper(AvCAST)_entry_ΔTniQ

    Plasmid
    #168138
    Purpose
    Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168138-plasmid-map-sequence-id-330692

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  49. pMS4: pHelper(AvCAST)_entry

    Plasmid
    #168137
    Purpose
    Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168137-plasmid-map-sequence-id-330973

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  50. pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

    Plasmid
    #168134
    Purpose
    Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168134-plasmid-map-sequence-id-330858

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Showing: 51 - 100 of 705 results