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We narrowed to 1,540 results for: SEU

Showing: 51 - 100 of 1540 results

  8. pSG069

    Plasmid
    #214260
    Purpose
    pJN105 with PaftsH2SD and PaFtsH2 ORF cloned between NheI/XbaI
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    tLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214260-plasmid-map-sequence-id-432474

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  11. pCPP5233

    Plasmid
    #128715
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi
    Insert
    shcM-hopM1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128715-plasmid-map-sequence-id-252574

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  13. pCPP3373

    Plasmid
    #128718
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(
    Insert
    hopQ1-1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous point mutation in the wobble position…
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128718-plasmid-map-sequence-id-252569

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  14. pSG068

    Plasmid
    #214259
    Purpose
    pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
    Use
    Tags
    Expression
    Bacterial
    Mutation
    18 nucleotide 5' of the start codon (Shine-D…
    Promoter
    araBAD promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214259-plasmid-map-sequence-id-432475

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  15. pSG059

    Plasmid
    #214262
    Purpose
    PaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    tLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
    Use
    Tags
    6x His-tag and 6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214262-plasmid-map-sequence-id-432470

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  16. pACB143

    Plasmid
    #222214
    Purpose
    For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    vanB(A24P)
    Use
    Tags
    N-terminal, thrombin-cleavable 6x His tag
    Expression
    Bacterial
    Mutation
    Changed Alanine 24 to Proline
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222214-plasmid-map-sequence-id-438176

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  18. pACB109

    Plasmid
    #222208
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (K10N)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Lys10 to Asn
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222208-plasmid-map-sequence-id-437369

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  19. pACB110

    Plasmid
    #222209
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (G258D)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Gly258 to Aspartate
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222209-plasmid-map-sequence-id-437372

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  20. pACB111

    Plasmid
    #222210
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(G76V)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed glycine 76 to valine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222210-plasmid-map-sequence-id-437376

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  21. pACB112

    Plasmid
    #222211
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(S11R)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Serine 11 to Arginine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222211-plasmid-map-sequence-id-437378

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  22. pACB113

    Plasmid
    #222212
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(W15C)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Tryptophan 15 to Cysteine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222212-plasmid-map-sequence-id-437381

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  25. pET20b_Pa-PirA

    Plasmid
    #128956
    Purpose
    expresses Pseudomonas aeruginosa PirA in E. coli
    Depositor
    James Naismith
    Article
    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24
    Insert
    ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
    Use
    Tags
    6xHis and pelB leader
    Expression
    Bacterial
    Mutation
    Signal peptide sequence has been removed
    Promoter
    T7
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128956-plasmid-map-sequence-id-254679

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  26. pSG071

    Plasmid
    #214257
    Purpose
    Hybrid PaFtsH1NFtsH2 protease: PaftsH2SD -PaftsH21-462-PaftsH1454-1920
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Hybrid PaFtsH protease PaFtsH1NFtsH2 of Pseudomonas aeruginosa SG17M
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…
    Promoter
    araBAD promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214257-plasmid-map-sequence-id-433134

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  27. pSG075

    Plasmid
    #214258
    Purpose
    Hybrid PaFtsH2NFtsH1 protease: PaftsH2SD -PaftsH11-453-PaftsH2463-1881 in pJN105
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Hybrid PaFtsH protease PaFtsH2NFtsH1 of Pseudomonas aeruginosa SG17M
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…
    Promoter
    araBAD promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214258-plasmid-map-sequence-id-432476

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  28. pSG086

    Plasmid
    #214263
    Purpose
    PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
    Use
    Tags
    6x His-tag
    Expression
    Bacterial
    Mutation
    substitution of 31 amino acids of FtsH2 (F124RRFA…
    Promoter
    T7 promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214263-plasmid-map-sequence-id-447733

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  34. pCPP5912

    Plasmid
    #128703
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi
    Insert
    shcE-avrE1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Three synonymous mutations Ala 362 (GCT-GCC), Ala…
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128703-plasmid-map-sequence-id-252488

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  35. pACB120

    Plasmid
    #222213
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(K10N, S11R, W15C, G76V, G258D)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Contains five amino acid mutations: K10N, S11R, W…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222213-plasmid-map-sequence-id-443111

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  39. pSB278 - pL0_pT16H2 (pro + 5U)

    Plasmid
    #203889
    Purpose
    T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d
    Insert
    T16H2 promoter and 5'UTR
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation of BpiI or BsaI cut site for MoClo domes…
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    203889-plasmid-map-sequence-id-404811

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  40. pUN1307 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pT3R

    Plasmid
    #203899
    Purpose
    Reporter plasmid for the T3R promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pT3R-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203899-plasmid-map-sequence-id-404833

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  41. pUN1308 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pNMT

    Plasmid
    #203900
    Purpose
    Reporter plasmid for the NMT promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pNMT-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203900-plasmid-map-sequence-id-404838

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  42. pUN1310 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pDAT

    Plasmid
    #203902
    Purpose
    Reporter plasmid for the DAT promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pDAT-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203902-plasmid-map-sequence-id-404844

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  43. pSB284 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pT16H2

    Plasmid
    #203896
    Purpose
    Reporter plasmid for the T16H2 promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pT16H2-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203896-plasmid-map-sequence-id-404824

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  44. pUN1305 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-p16OMT

    Plasmid
    #203897
    Purpose
    Reporter plasmid for the 16OMT promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    p16OMT-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203897-plasmid-map-sequence-id-404828

    Enlarge

  45. pUN1306 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pT3O

    Plasmid
    #203898
    Purpose
    Reporter plasmid for the T3O promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pT3O-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203898-plasmid-map-sequence-id-404831

    Enlarge

  47. pUN1213 - pL0_p16OMT (pro + 5U)

    Plasmid
    #203890
    Purpose
    16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d
    Insert
    16OMT promoter and 5'UTR
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation of BpiI and/or BsaI cut sites for MoClo …
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    203890-plasmid-map-sequence-id-404813

    Enlarge

  48. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

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Showing: 51 - 100 of 1540 results