We narrowed to 115 results for: hpgk promoter

1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide3

   Plasmid

   #125434

   Purpose

   CRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide2

   Plasmid

   #125433

   Purpose

   CRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR PHF21A-TSS-guide1

   Plasmid

   #125432

   Purpose

   CRISPR-mediated repression of PHF21A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide4

   Plasmid

   #125431

   Purpose

   CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide4

   Plasmid

   #125423

   Purpose

   CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide3

   Plasmid

   #125422

   Purpose

   CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide1

   Plasmid

   #125420

   Purpose

   CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide3

   Plasmid

   #125418

   Purpose

   CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide2

   Plasmid

   #125417

   Purpose

   CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4A-TSS-guide1

    Plasmid

    #125416

    Purpose

    CRISPR-mediated repression of C2CD4A. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide4

    Plasmid

    #125415

    Purpose

    CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide1

    Plasmid

    #125412

    Purpose

    CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide2

    Plasmid

    #118178

    Purpose

    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide3

    Plasmid

    #118179

    Purpose

    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide2

    Plasmid

    #118182

    Purpose

    CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide1

    Plasmid

    #118181

    Purpose

    CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide1

    Plasmid

    #118173

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide2

    Plasmid

    #118174

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide4

    Plasmid

    #118180

    Purpose

    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide3

    Plasmid

    #118183

    Purpose

    CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide4

    Plasmid

    #118176

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide4

    Plasmid

    #118172

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide1

    Plasmid

    #118177

    Purpose

    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188744

    Purpose

    Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO.1-GST-EGFP-GPIDAF

    Plasmid

    #213716

    Purpose

    For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an hPGK promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLKO.1-GST-EGFP-GPIBY55

    Plasmid

    #213717

    Purpose

    For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 driven by an hPGK promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO.1-GST-EGFP-GPICEAM7

    Plasmid

    #213718

    Purpose

    For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an hPGK promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1-TST-EGFP-GPIDAF

    Plasmid

    #213708

    Purpose

    For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    Tags

    Twin-strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO.1-TST-EGFP-GPIBY55

    Plasmid

    #213707

    Purpose

    For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    Tags

    Twin-strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

    Plasmid

    #194925

    Purpose

    mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

    Depositor

    Hao Xu

    Article

    Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDUAL OCLN (GFP)

    Plasmid

    #86982

    Purpose

    Lentiviral expression construct encoding Occludin and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    OCLN (OCLN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDUAL SRBI (GFP)

    Plasmid

    #86979

    Purpose

    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    SCARB1 (SCARB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222502

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDUAL CLDN (GFP)

    Plasmid

    #86981

    Purpose

    Lentiviral expression construct encoding Claudin-1 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    CLDN1 (CLDN1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDUAL CD81 (GFP)

    Plasmid

    #86980

    Purpose

    Lentiviral expression construct encoding CD81 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    CD81 (CD81 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 5` CC: Puro – loxP – GFP-C

    Plasmid

    #219563

    Purpose

    5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    hPGK-promoter_PuroR_2A_loxP_GFP-C
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TRE3GV-SpdCas9-Dnmt3A/3L-V1

    Plasmid

    #222503

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid

    #222505

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TRE3GV-SpdCas9-Dnmt3A/3L-V3

    Plasmid

    #222506

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E and E814…

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid

    #222508

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; C706A and R832…

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLVPT-tTR-KRAB

    Plasmid

    #11642

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLVPT-rtTR-KRAB-2SM2

    Plasmid

    #11652

    Purpose

    Tet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, rtTR-KRAB-2SM2, Tet-off
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMALB

    Plasmid

    #161784

    Purpose

    Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bidirectional minhCMV and hPGK

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMAL

    Plasmid

    #161783

    Purpose

    Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and GFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bidirectional minhCMV and hPGK

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUC57-PB-PGK-tetR-KRAB-2A-Puro

    Plasmid

    #129536

    Purpose

    PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    PB-PGK-tetR-KRAB-2A-Puro
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LV-LIN28A

    Plasmid

    #117057

    Purpose

    Overexpression of mouse LIN28A

    Depositor

    Michele De Palma

    Article

    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

    Insert

    LIN28A (Lin28a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

    Plasmid

    #129537

    Purpose

    PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    tetR-3xFlag-HA-2A-Puro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLKO.1-TRC.mKO2

    Plasmid

    #85208

    Purpose

    Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid

    #211821

    Purpose

    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter, U6 promoter and hPGK pr…

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

    Plasmid

    #211815

    Purpose

    lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9, tracrRNA and puromycin selection gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a promoter, U6 promoter, and hPGK promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge