1. pCAGGS-flpE-puro

   Plasmid

   #20733

   Purpose

   Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   FLPe recombinase
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. CycA-Venus-Flag (1305)

   Plasmid

   #39853

   Depositor

   Jonathon Pines

   Article

   Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

   Insert

   Cyclin A (CCNA2 Human)
   

   Use

   Tags

   Flag and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pBS7

   Plasmid

   #83774

   Purpose

   Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Venus
   

   Use

   Tags

   Venus

   Expression

   Yeast

   Mutation

   F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDH6

   Plasmid

   #83777

   Purpose

   Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   YFP
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   S65G, V68L, Q69K, S72A, T203Y mutations added.

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDH5

   Plasmid

   #83775

   Purpose

   Useful for tagging yeast proteins with C-terminal YFP tag; his selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   YFP
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…

   Promoter

   Available since

   Oct. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1_Puro_hPGK1_EGFP_Donor

   Plasmid

   #178088

   Purpose

   HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1_Puro_hPGK1_EBFP_Donor

   Plasmid

   #178089

   Purpose

   HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EBFP (T65S/F145Y)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NPM1_mNeonGreen_Donor

   Plasmid

   #178093

   Purpose

   HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   NPM1-mNeonGreen HDR donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LMNA_mScarlet-I_Donor

   Plasmid

   #178092

   Purpose

   HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   LMNA-mScarlet-I HDR donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDH3

   Plasmid

   #83789

   Purpose

   Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   CFP
   

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   F64L, Y66W, N146I, M153T, V163A, N164H mutations …

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330_ATP1A1_G7

    Plasmid

    #173204

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP1A1_T804N_hPGK1_mScarlet-I_Donor

    Plasmid

    #173207

    Purpose

    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1-T804N mScarlet-I donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK1

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid

    #178087

    Purpose

    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    mScarlet-I mTOR signaling indicator (TOSI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ATP1A1_G3_EBFP_Nick_Dual_sgRNA

    Plasmid

    #178094

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EBFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

    Plasmid

    #178095

    Purpose

    Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + EGFP nick sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7 cyclin A1-164 venus cks1 (2196)

    Plasmid

    #61839

    Purpose

    expression of N terminal cyclin A and cks1

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

    Insert

    cyclin A (CCNA2 Human)
    

    Use

    In vitro transcription

    Tags

    Venus

    Expression

    Mutation

    L2V

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP

    Plasmid

    #139283

    Purpose

    Express hChR2(H134R)-P2A-EYFP in a Cre-dependent manner

    Depositor

    Mingshan Xue

    Article

    Chen et al Elife. 2020 Feb 19;9. pii: 48705. doi: 1

    Insert

    hChR2(H134R)-P2A-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATP1A1_G3_Dual_sgRNA

    Plasmid

    #173202

    Purpose

    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid

    #178091

    Purpose

    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ATP1A1_G2_Dual_sgRNA

    Plasmid

    #173201

    Purpose

    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_ATP1A1_G7_Dual_sgRNA

    Plasmid

    #173206

    Purpose

    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge