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pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]

Plasmid

#159900

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mcherry | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Pmlc-1

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

Plasmid

#159896

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

Plasmid

#193854

Purpose

Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

5' Cbr-unc-119(+) + 5' mNeonGreen

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Cbr-unc-119p

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]

Plasmid

#159895

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

Plasmid

#159898

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

Plasmid

#159795

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Plasmid

#159794

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

Plasmid

#159817

Purpose

Positive control for germline GFP expression from simple array in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPK719

Plasmid

#38149

Depositor

Patricia Kuwabara

Insert

cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)

Use

Recombineering

Tags

Expression

Worm

Mutation

Promoter

unc-119

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pMLS291

Plasmid

#73724

Purpose

SapTrap mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS252

Plasmid

#73720

Purpose

SapTrap GFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS286

Plasmid

#73723

Purpose

Saptrap tagRFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

tagRFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS271

Plasmid

#73722

Purpose

SapTrap SNAPtag + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Snaptag + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS254

Plasmid

#73721

Purpose

SapTrap Halotag + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Halotag + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS292

Plasmid

#73725

Purpose

SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS236

Plasmid

#73684

Purpose

SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS235

Plasmid

#73683

Purpose

SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS338

Plasmid

#73719

Purpose

SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Unc-32::GFP(Cbr-unc-119)

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pNB769

Plasmid

#60138

Purpose

Red click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Use

Tags

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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