We narrowed to 1,732 results for: FAST

1. pFastBacMam6_C_3C_His8_Stop

   Plasmid

   #178810

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Tags

   3C, His8, Stop codon

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILFPmTagBFP2FrFAST

   Plasmid

   #223839

   Purpose

   Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

   Insert

   pTEF1>mTagBFP2>FrFAST
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. ER-frNFAST

   Plasmid

   #233599

   Purpose

   Expression of frNFAST on the ER membrane

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   frNFAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PM-frNFAST

   Plasmid

   #233602

   Purpose

   Expression of frNFAST on the PM facing the cytosol

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   frNFAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBacHTa_LYN

   Plasmid

   #224244

   Purpose

   Expression of human LYN Kinase in insect cells (SF9 cells)

   Depositor

   Andrew Mesecar

   Insert

   lyn (LYN Human)
   

   Tags

   6xHis-TEV

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBacHTa_SYK

   Plasmid

   #224246

   Purpose

   Expression of human SYK Kinase in insect cells (SF9 cells)

   Depositor

   Andrew Mesecar

   Insert

   syk (SYK Human)
   

   Tags

   6xHis-TEV

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac HT JS-Rab10wt

   Plasmid

   #135557

   Purpose

   Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Rab10 (Rab10 Mouse)
   

   Tags

   6xHis tag, TEV site

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICSFAST-ER-PO-P2A

   Plasmid

   #214894

   Purpose

   Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PO-P2A
   

   Tags

   none

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPLICSFAST-ER-MT-P2A

   Plasmid

   #214892

   Purpose

   Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-MT-P2A
   

   Tags

   none

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-SPLICSs-cFAST10

    Plasmid

    #210431

    Purpose

    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    ER-SPLICSs-cFAST10
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-pGC-A

    Plasmid

    #186626

    Purpose

    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

    Depositor

    Debra Hansen

    Article

    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

    Insert

    NPR1 (NPR1 Human)
    

    Tags

    hemagglutinin (HA) signal peptide + His10-tag + T…

    Expression

    Insect

    Mutation

    deleted amino acids 1-32

    Promoter

    polyhedring

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPLICSFAST-LY-MT-P2A

    Plasmid

    #214897

    Purpose

    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LY-MT-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRE-Fast-FT

    Plasmid

    #31913

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Fast-FT
    

    Expression

    Mammalian

    Mutation

    M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

    Promoter

    PTight Tet-responsive PminCMVd

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac-CHD7

    Plasmid

    #170141

    Purpose

    Expresses CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Tags

    6xHis and FLAG

    Expression

    Insect

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac HT JS-Munc18c

    Plasmid

    #135555

    Purpose

    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18c
    

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-iGlu3Fast

    Plasmid

    #226500

    Purpose

    Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release

    Depositor

    Katalin Torok

    Article

    Tran et al bioRxiv, August 30th, 2024

    Insert

    iGluSnFR3 S72T
    

    Tags

    IgK, Myc tag, and PDGFR Beta TM domain

    Expression

    Mammalian

    Mutation

    S72T

    Promoter

    CMV

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG654 pFAST

    Plasmid

    #172861

    Purpose

    Expresses pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    pFAST
    

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPLICSFAST-PO-MT-P2A

    Plasmid

    #214899

    Purpose

    Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-PO-MT-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPLICSFAST-NU-MT-P2A

    Plasmid

    #214898

    Purpose

    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-NU-MT-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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