We narrowed to 234 results for: G1

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pRSF-G1(7AW)RS

Plasmid

#207516

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6CNW)RS

Plasmid

#222873

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7CNW)RS

Plasmid

#222874

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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msHook2 g1 lentiCRISPRv2-mCherry

Plasmid

#218652

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM165 g1 LentiCRISPRv2-mCherry

Plasmid

#218660

Purpose

Knockout vector for human TMEM165

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM165 (TMEM165 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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GOLIM4 g1 LentiCRISPRv2-mCherry

Plasmid

#218662

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-POLB-KO-g1

Plasmid

#176090

Purpose

Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

POLB sgRNA #1 (POLB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6/7FTrp)RS

Plasmid

#207621

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4/5FTrp)RS

Plasmid

#207620

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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PB-EEA-g1-PGK-Puro

Plasmid

#102908

Purpose

PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-guide1-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT FLAG-G1 PylRS

Plasmid

#154768

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

Plasmid

#218654

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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msPlekha8 (msFapp2) g1 lentiCRISPRv2-opti

Plasmid

#218650

Purpose

Knockout vector for mouse Plekha8 (Fapp2)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Plekha8 (Plekha8 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msPlekha3 (msFapp1) g1 lentiCRISPRv2-opti

Plasmid

#218648

Purpose

Knockout vector for mouse Plekha3 (Fapp1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Plekha3 (Plekha3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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PLEKHA8 (FAPP2) g1 lentiCRISPRv2-opti

Plasmid

#218658

Purpose

Knockout vector for human PLEKHA8 (FAPP2)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

PLEKHA8 (PLEKHA8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti

Plasmid

#218656

Purpose

Knockout vector for human PLEKHA3 (FAPP1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

PLEKHA3 (PLEKHA3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP8-Myc-FLAG (G418)

Plasmid

#86850

Purpose

mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Plasmid

#222994

Purpose

Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP6-Myc-FLAG (puromycin)

Plasmid

#86851

Purpose

mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP6 (ATP6 Human)

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon corrected

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT FLAG-G1 PylRS Y125A

Plasmid

#154769

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Mutation

Y125A

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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