We narrowed to 1,221 results for: POLI

1. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_WT POLI

   Plasmid

   #131228

   Purpose

   Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Coding sequence has been codon optimised for expr…

   Promoter

   CMV enhancer + CMV promoter

   Available Since

   Dec. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-DDK_UBM1*2* POLI

   Plasmid

   #131232

   Purpose

   Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains inactivating mutations in both ubiquitin…

   Promoter

   CMV enhancer + CMV promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pESC-URA-hPOLISc

   Plasmid

   #60819

   Purpose

   to express human Pol iota recoded to yeast codons in yeast

   Depositor

   Youri Pavlov

   Article

   Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

   Insert

   POLI (POLI Human)
   

   Tags

   c-myc

   Expression

   Yeast

   Mutation

   optimized to yeast codon usage

   Promoter

   GAL1

   Available Since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. apoeb (apolipoprotein Eb)_L (OZ529)

   Plasmid

   #27206

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting apoeb (apoeb Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

6. apoeb (apolipoprotein Eb)_R (OZ530)

   Plasmid

   #27207

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting apoeb (apoeb Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. RS314-U6(PolII-Lsm)

   Plasmid

   #200772

   Purpose

   Pol II expression of U6 Lsm

   Depositor

   Aaron Hoskins

   Article

   Lipinski et al RNA. 2022 Dec;28(12):1606-1620. doi: 10.

   Insert

   Gal-SNR6-II
   

   Expression

   Bacterial and Yeast

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AN-DDK_S433A POLI

   Plasmid

   #131235

   Purpose

   Mammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-AN-DDK_S497A POLI

   Plasmid

   #131236

   Purpose

   Mammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains a point mutation converting Pol iota ser…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-AN-DDK_S520A POLI

    Plasmid

    #131237

    Purpose

    Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-AN-DDK_S580A POLI

    Plasmid

    #131238

    Purpose

    Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a point mutation converting Pol iota ser…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_3KA POLI

    Plasmid

    #131239

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert lysine resi…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEvolvR-enCas9-PolI3M-TBD

    Plasmid

    #113077

    Purpose

    Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI3M-TBD
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-HA_aa 1-420 POLI

    Plasmid

    #201582

    Purpose

    Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-420 of DNA polymerase iota

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJRM919_WT aa 1-419 His POLI

    Plasmid

    #201445

    Purpose

    Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 1-449 of DNA polymerase iot…

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-AN-DDK_RKK/AAA POLI

    Plasmid

    #131240

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert arginine re…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-AN-DDK_3KA + S580A POLI

    Plasmid

    #131241

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert Pol iota se…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-AN-DDK_1-590 POLI

    Plasmid

    #131233

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV6-AN-DDK_1-420 POLI

    Plasmid

    #131234

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge